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Literature summary extracted from

  • Yu, S.; Andreassen, M.; Lundt, I.
    Enzymatic production of microthecin by aldos-2-ulose dehydratase from 1,5-anhydro-D-fructose and stability studies of microthecin (2008), Biocatal. Biotransform., 26, 169-176.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.110 Microthecin is found to be most stable in de-ionized water at a pH around 7.0 and stable in freeze-dried form. Under acidic conditions microthecin is degraded to 2’-furyl-2-hydroxymethylketone (FHMK). Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.110 1,5-Anhydro-D-fructose substrate inhibition to AUDH at a concentration up to 10% (w/v) Phanerodontia chrysosporium
4.2.1.110 microthecin product inhibition to AUDH at a concentration up to 10% (w/v) Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.110 4.9
-
 glucosone
-
 Phanerodontia chrysosporium
4.2.1.110 5.4
-
 1,5-Anhydro-D-fructose
-
 Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.110 glucosone Phanerodontia chrysosporium 2-keto glucose cortalcerone + H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.2.1.110 additional information the best solvent for AUDH-catalysed microthecin reaction is de-ionized water Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.110 Phanerodontia chrysosporium P84193
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.110 1,5-anhydro-D-fructose
-
 Phanerodontia chrysosporium 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O microthecin + H2O ?
4.2.1.110 glucosone 2-keto glucose Phanerodontia chrysosporium cortalcerone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.110 aldos-2-ulose dehydratase bifunctional enzyme Phanerodontia chrysosporium
4.2.1.110 AUDH
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.110 24
-
 at temperatures higher than 24°C there is increased degradation of microthecin Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.110 additional information
-
 the reaction medium acidifies by itself due to unknown events related to the AUDH catalysed reaction Phanerodontia chrysosporium
4.2.1.110 5
-
 at a pH lower than 5.0, the reaction rate is slower and there is a tendency for increased formation of degradation products (FHMK), at a pH higher than 5.5, there is a tendency for more APT dihydrate formation Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.110 additional information
-
 at a pH lower than 5.0, the reaction rate is slower and there is a tendency for increased formation of degradation products (FHMK), at a pH higher than 5.5, there is a tendency for more APT dihydrate formation Phanerodontia chrysosporium