EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.14 | expression of wild-type and truncation mutant enzymes in Escherichia coli BL21(DE3) | Aeromonas caviae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.14 | additional information | generation of truncated mutant AcD1Chi-AK606 | Aeromonas caviae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.14 | 3.62 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae | |
3.2.1.14 | 5.35 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae | |
3.2.1.14 | 53.33 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae | |
3.2.1.14 | 57.83 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.14 | extracellular | - |
Aeromonas caviae | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 66000 | - |
x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE | Aeromonas caviae |
3.2.1.14 | 97000 | - |
x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE | Aeromonas caviae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.14 | Aeromonas caviae | - |
- |
- |
3.2.1.14 | Aeromonas caviae D1 | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.14 | cell culture | the organism produces an extracellular chitinase when grown on the chitin as a sole carbon source | Aeromonas caviae | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.14 | 4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae | ? | - |
? | |
3.2.1.14 | 4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae D1 | ? | - |
? | |
3.2.1.14 | 4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae | ? | - |
? | |
3.2.1.14 | 4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae D1 | ? | - |
? | |
3.2.1.14 | alpha-chitin + H2O | insoluble substrate | Aeromonas caviae | ? | - |
? | |
3.2.1.14 | alpha-chitin + H2O | insoluble substrate | Aeromonas caviae D1 | ? | - |
? | |
3.2.1.14 | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae | ? | - |
? | |
3.2.1.14 | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Aeromonas caviae D1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.14 | ? | x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE | Aeromonas caviae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.14 | AcD1ChiA | - |
Aeromonas caviae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 50 | - |
- |
Aeromonas caviae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.14 | 0.0007 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae | |
3.2.1.14 | 0.0012 | - |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae | |
3.2.1.14 | 0.0047 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme | Aeromonas caviae | |
3.2.1.14 | 0.006 | - |
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc | pH 6.0, 50°C, recombinant His-tagged wild-type enzyme | Aeromonas caviae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 6 | - |
- |
Aeromonas caviae |