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Literature summary extracted from

  • Goenrich, M.; Thauer, R.K.; Yurimoto, H.; Kato, N.
    Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis (2005), Arch. Microbiol., 184, 41-48.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.43 expressed in Escherichia coli BL21 (DE3) pLysS cells Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 42000
-
 SDS-PAGE Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Methanosarcina barkeri
-
-
-
4.1.2.43 Methanosarcina barkeri Fusaro / DSM 804
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 DEAE-Sephacel column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 4.4
-
 hexulose-6-phosphate synthase activity of the gene product Fae-Hps, at 30°C, pH 7.0 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methanosarcina barkeri D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methanosarcina barkeri Fusaro / DSM 804 D-arabino-hex-3-ulose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 Fae-Hps protein with hexulose-6-phosphate synthase and formaldehyde activating enzyme activities Methanosarcina barkeri
4.1.2.43 hexulose-6-phosphate synthase
-
 Methanosarcina barkeri