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Literature summary extracted from

  • Voynova, N.E.; Fu, Z.; Battaile, K.P.; Herdendorf, T.J.; Kim, J.J.; Miziorko, H.M.
    Human mevalonate diphosphate decarboxylase: characterization, investigation of the mevalonate diphosphate binding site, and crystal structure (2008), Arch. Biochem. Biophys., 480, 58-67.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.33 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.33 vapour diffusion method, with 0.1 M MES buffer pH 6.5, 20% PEG 5000 MME, 0.1 M NaCl, 1 mM dithiothreitol, and 0.2 M ammonium sulfate Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.33 N17A mutant exhibits a 15fold inflation in Km for 5-diphosphomevalonate Homo sapiens
4.1.1.33 R161Q mutant exhibits an about 1000fold diminution in specific activity, while binding the fluorescent substrate analog, TNP-ATP, comparably to wild type enzyme Homo sapiens
4.1.1.33 S162A mutant shows reduced activity with 2fold decrease in Vm and 4 to 7fold increases in substrate Km values Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.33 6-fluoromevalonate 5-diphosphate competitive inhibitor with respect to 5-diphosphomevalonate Homo sapiens
4.1.1.33 diphosphoglycolyl proline competitive inhibitor with respect to 5-diphosphomevalonate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.33 0.0289
-
 5-diphosphomevalonate wild type enzyme, at 30°C Homo sapiens
4.1.1.33 0.1257
-
 5-diphosphomevalonate mutant enzyme S162A, at 30°C Homo sapiens
4.1.1.33 0.425
-
 5-diphosphomevalonate mutant enzyme N17A, at 30°C Homo sapiens
4.1.1.33 0.69
-
 ATP wild type enzyme, at 30°C Homo sapiens
4.1.1.33 4.9
-
 ATP mutant enzyme S162A, at 30°C Homo sapiens
4.1.1.33 7.7
-
 ATP mutant enzyme N17A, at 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.33 Mg2+ 20 mM, required for activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.33 44000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.33 Homo sapiens P53602
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.33 Ni-Sepharose resin chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.33 ATP + 5-diphosphomevalonate
-
 Homo sapiens ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.33 dimer x-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.33 MDD
-
 Homo sapiens
4.1.1.33 mevalonate diphosphate decarboxylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.33 0.0022
-
 5-diphosphomevalonate mutant enzyme R161Q, at 30°C Homo sapiens
4.1.1.33 0.2
-
 5-diphosphomevalonate mutant enzyme N17A, at 30°C Homo sapiens
4.1.1.33 2.1
-
 5-diphosphomevalonate mutant enzyme S162A, at 30°C Homo sapiens
4.1.1.33 4.5
-
 5-diphosphomevalonate wild type enzyme, at 30°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.33 ATP
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.33 0.000062
-
 6-fluoromevalonate 5-diphosphate wild type enzyme Homo sapiens
4.1.1.33 0.0023
-
 diphosphoglycolyl proline wild type enzyme Homo sapiens