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Literature summary extracted from

  • Hevel, J.M.; Pande, P.; Viera-Oveson, S.; Sudweeks, T.J.; Jaffree, L.S.; Hansen, C.M.; Ayling, J.E.
    Determinants of oligomerization of the bifunctional protein DCoHalpha and the effect on its enzymatic and transcriptional coactivator activities (2008), Arch. Biochem. Biophys., 477, 356-362.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.96 expression in Escherichia coli BL21(DE3)pLysS Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.96 additional information 3 residues, Asn61, Gln45, and Lys98 in DCoHalpha play a role in oligomeric flexibility, which enables DCoHalpha to more readily interact with HNF1alpha and increase DNA binding Homo sapiens
4.2.1.96 N61D The decreased ability of the N61D mutant to affect HNF1alpha-dependent DNA binding is likely a direct result of altered quaternary structure. Homo sapiens
4.2.1.96 N61D/Q45R/K98Q site-directed mutagenesis, triple DCoHa mutant (Q45R/K98Q/N61D) is unable to affect HNF1alpha-dependent DNA binding in vitro. Homo sapiens
4.2.1.96 Q45R/K98Q mutant Q45R/K98Q is not able to affect HNF1alpha-dependent DNA binding in vitro Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.96 0.004
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin wild-type Homo sapiens
4.2.1.96 0.0044
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin Q45R/K98Q Homo sapiens
4.2.1.96 0.0052
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D Homo sapiens
4.2.1.96 0.0053
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D/Q45R/K98Q Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.96 Homo sapiens Q9H0N5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.96 at 4°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.2.1.96 mutant proteins require the inclusion of 10% glycerol in the storage buffer to maintain solubility Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.96 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin
-
 Homo sapiens (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O
-
 ?
4.2.1.96 additional information DCoHalpha is coactivator of HNF1alpha-depndent transcription Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.96 dimer tetramer-dimer equilibrium at lower concentration, wild-type Homo sapiens
4.2.1.96 tetramer high concentration, wild-type Homo sapiens
4.2.1.96 tetramer mutant enzyme N61D/Q45R/K98Q shows no evidence of a dimeric spiecies Homo sapiens
4.2.1.96 tetramer mutant enzyme Q45R/K98Q shows no evidence of a dimeric spiecies Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.96 4a-Hydroxytetrahydrobiopterin dehydratase
-
 Homo sapiens
4.2.1.96 DCoHalpha
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.96 23
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D Homo sapiens
4.2.1.96 23
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D/Q45R/K98Q Homo sapiens
4.2.1.96 23
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin Q45R/K98Q Homo sapiens
4.2.1.96 24
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin wild-type Homo sapiens