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Literature summary extracted from

  • Kohl, A.C.; Kerr, R.G.
    Identification and characterization of the pseudopterosin diterpene cyclase, elisabethatriene synthase, from the marine gorgonian, Pseudopterogorgia elisabethae (2004), Arch. Biochem. Biophys., 424, 97-104.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.41 diethyl diphosphate
-
 Antillogorgia elisabethae
4.2.3.41 additional information no inhibition by 0.05 mM N-ethylmaleimide Antillogorgia elisabethae
4.2.3.41 oxo(phenyl)acetaldehyde
-
 Antillogorgia elisabethae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.41 0.007
-
 geranylgeranyl diphosphate 29°C Antillogorgia elisabethae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.41 Mg2+ the enzyme can not catalyze the cyclization reaction in the absence of Mg2+, saturating concentration is 1.5 mM, Km-value: 0.78 mM. At a concentration of 2 mM, Mg2+ supports the cyclization reaction most efficiently. When substituting Mn2+ for Mg2+, approximately one-third of the elisabethatriene synthase activity is observed Antillogorgia elisabethae
4.2.3.41 Mn2+ at a concentration of 2 mM, Mg2+ supports the cyclization reaction most efficiently. When substituting Mn2+ for Mg2+, approximately one-third of the elisabethatriene synthase activity is observed Antillogorgia elisabethae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.41 47000
-
 gel filtration Antillogorgia elisabethae

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.41 Antillogorgia elisabethae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.41 partial Antillogorgia elisabethae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.41 geranylgeranyl diphosphate
-
 Antillogorgia elisabethae elisabethatriene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.41 monomer 1 * 47000, SDS-PAGE Antillogorgia elisabethae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.41 7.1
-
 highest activity in phosphate buffer Antillogorgia elisabethae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.41 Antillogorgia elisabethae isoelectric focusing, pH-range 5-8
-
 5.1

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.3.41 4.5
-
 29°C Antillogorgia elisabethae oxo(phenyl)acetaldehyde
4.2.3.41 4.9
-
 29°C Antillogorgia elisabethae diethyl diphosphate