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Literature summary extracted from
- Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis (1994), Arch. Biochem. Biophys., 308, 258-266.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.74 | additional information | not inhibited by 90% CO | Abies grandis | |
| 1.14.14.144 | Ancymidol | - |
Abies grandis |  |
| 1.14.14.144 | clotrimazole | - |
Abies grandis | |
| 1.14.14.144 | CO | blue light reversible inhibition | Abies grandis | |
| 1.14.14.144 | cytochrome c | - |
Abies grandis | |
| 1.14.14.144 | Metyrapone | - |
Abies grandis | |
| 1.14.14.144 | miconazole | - |
Abies grandis | |
| 1.14.14.145 | Ancymidol | - |
Abies grandis | |
| 1.14.14.145 | clotrimazole | - |
Abies grandis | |
| 1.14.14.145 | CO | blue light reversible inhibition | Abies grandis | |
| 1.14.14.145 | cytochrome c | - |
Abies grandis | |
| 1.14.14.145 | Metyrapone | - |
Abies grandis | |
| 1.14.14.145 | miconazole | - |
Abies grandis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.144 | 0.0042 | - |
abietadiene | pH 7.5, 32°C | Abies grandis | |
| 1.14.14.145 | 0.003 | - |
abietadienol | pH 7.5, 32°C | Abies grandis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.2.1.74 | cytoplasm | - |
Abies grandis | 5737 | - |
| 1.14.14.144 | microsome | - |
Abies grandis | - |
- |
| 1.14.14.144 | microsome | - |
Pinus contorta | - |
- |
| 1.14.14.145 | microsome | - |
Abies grandis | - |
- |
| 1.14.14.145 | microsome | - |
Pinus contorta | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.74 | abietadienal + H2O + NAD+ | Abies grandis | last step of abietic acid biosynthesis. Abietic acid is the principle component of conifer resin as a defense secretion against insect and pathogen attack | abietate + NADH + H+ | - |
? | |
| 1.14.14.144 | abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | Pinus contorta | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.144 | abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | Abies grandis | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 | Pinus contorta | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis | abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 | Abies grandis | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant | abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.74 | Abies grandis | - |
Lindl. | - |
| 1.14.14.144 | Abies grandis | - |
Lindl. | - |
| 1.14.14.144 | Pinus contorta | - |
Douglas var. latifolia Engelman | - |
| 1.14.14.145 | Abies grandis | - |
Lindl. | - |
| 1.14.14.145 | Pinus contorta | - |
Douglas var. latifolia Engelman | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.2.1.74 | stem | cell-free stem extracts | Abies grandis | - |
| 1.14.14.144 | stem | cell-free stem extracts | Abies grandis | - |
| 1.14.14.144 | stem | cell-free stem extracts | Pinus contorta | - |
| 1.14.14.145 | stem | cell-free stem extracts | Abies grandis | - |
| 1.14.14.145 | stem | cell-free stem extracts | Pinus contorta | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.74 | abietadienal + H2O + NAD+ | - |
Abies grandis | abietate + NADH + H+ | - |
? | |
| 1.2.1.74 | abietadienal + H2O + NAD+ | last step of abietic acid biosynthesis. Abietic acid is the principle component of conifer resin as a defense secretion against insect and pathogen attack | Abies grandis | abietate + NADH + H+ | - |
? | |
| 1.14.14.144 | abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus contorta | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.144 | abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis | Pinus contorta | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.144 | abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant | Abies grandis | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis | Pinus contorta | abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant | Abies grandis | abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.1.74 | abieta-7-13-dien-18-al dehydrogenase | - |
Abies grandis |
| 1.14.14.144 | abietadiene-18-hydroxylase | - |
Abies grandis |
| 1.14.14.144 | abietadiene-18-hydroxylase | - |
Pinus contorta |
| 1.14.14.145 | abieta-7,13-dien-18-ol hydroxylase | - |
Abies grandis |
| 1.14.14.145 | abieta-7,13-dien-18-ol hydroxylase | - |
Pinus contorta |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.74 | 32 | - |
assay at | Abies grandis |
| 1.14.14.144 | 32 | - |
assay at | Abies grandis |
| 1.14.14.144 | 32 | - |
assay at | Pinus contorta |
| 1.14.14.145 | 32 | - |
assay at | Abies grandis |
| 1.14.14.145 | 32 | - |
assay at | Pinus contorta |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.74 | 7.6 | - |
assay at | Abies grandis |
| 1.14.14.144 | 7.5 | 7.6 | - |
Abies grandis |
| 1.14.14.145 | 7.5 | 7.6 | - |
Abies grandis |
| 1.14.14.145 | 7.6 | - |
assay at | Pinus contorta |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.144 | 7 | 8 | 60-70% of maximal activity at pH 7.0 and 8.0 | Abies grandis |
| 1.14.14.145 | 7 | 8 | 60-70% of maximal activity at pH 7.0 and 8.0 | Abies grandis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.74 | NAD+ | - |
Abies grandis | |
| 1.14.14.144 | cytochrome P450 | cytochrome P-450-dependent monooxygenase | Abies grandis | |
| 1.14.14.144 | FAD | omission of flavins results in measurable reduction in both hydroxylation activities | Abies grandis | |
| 1.14.14.144 | FMN | omission of flavins results in measurable reduction in both hydroxylation activities | Abies grandis | |
| 1.14.14.145 | cytochrome P450 | cytochrome P-450-dependent monooxygenase | Abies grandis | |
| 1.14.14.145 | FAD | omission of flavins results in measurable reduction in both hydroxylation activities | Abies grandis | |
| 1.14.14.145 | FMN | omission of flavins results in measurable reduction in both hydroxylation activities | Abies grandis | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.144 | 0.0005 | - |
pH 7.5, 32°C | Abies grandis | clotrimazole | |
| 1.14.14.144 | 0.0009 | - |
pH 7.5, 32°C | Abies grandis | cytochrome c | |
| 1.14.14.144 | 0.005 | - |
pH 7.5, 32°C | Abies grandis | Metyrapone | |
| 1.14.14.144 | 0.0093 | - |
pH 7.5, 32°C | Abies grandis | Ancymidol | |
| 1.14.14.144 | 0.307 | - |
pH 7.5, 32°C | Abies grandis | miconazole | |
| 1.14.14.145 | additional information | - |
no inhibition detected by ancymidol at 0.2 mM | Abies grandis | additional information | |
| 1.14.14.145 | 0.0013 | - |
pH 7.5, 32°C | Abies grandis | clotrimazole | |
| 1.14.14.145 | 0.004 | - |
pH 7.5, 32°C | Abies grandis | cytochrome c | |
| 1.14.14.145 | 0.005 | - |
pH 7.5, 32°C | Abies grandis | Metyrapone | |
| 1.14.14.145 | 0.307 | - |
pH 7.5, 32°C | Abies grandis | miconazole | |
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