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Literature summary extracted from

  • Phithakrotchanakoon, C.; Daduang, R.; Thamchaipenet, A.; Wangkam, T.; Srikhirin, T.; Eurwilaichitr, L.; Champreda, V.
    Heterologous expression of polyhydroxyalkanoate depolymerase from Thermobifida sp. in Pichia pastoris and catalytic analysis by surface plasmon resonance (2009), Appl. Microbiol. Biotechnol., 82, 131-140.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 expressed as a C-terminal His6-tagged fusion in Pichia pastoris Thermobifida sp. BCC23166

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.75 0.79
-
 p-nitrophenyl butyrate
-
 Thermobifida sp. BCC23166

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 45640
-
 unglycosylated form of PhaZ-Th, SDS-PAGE Thermobifida sp. BCC23166
3.1.1.75 61000
-
 expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE Thermobifida sp. BCC23166
3.1.1.75 70000
-
 expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE Thermobifida sp. BCC23166

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Thermobifida sp. BCC23166 B3VS85 fragment
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.75 glycoprotein expressed as two glycosylated forms with apparent molecular weights of 61 and 70 kDa, SDS-PAGE Thermobifida sp. BCC23166

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 recombinant enzyme with a C-terminal His6 tag, Ni-Sepharose Fast Flow column, SDS-PAGE Thermobifida sp. BCC23166

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 p-nitrophenyl acetate + H2O
-
 Thermobifida sp. BCC23166 4-nitrophenol + acetate
-
 ?
3.1.1.75 p-nitrophenyl butyrate + H2O
-
 Thermobifida sp. BCC23166 4-nitrophenol + butyrate
-
 ?
3.1.1.75 p-nitrophenyl myristate + H2O
-
 Thermobifida sp. BCC23166 4-nitrophenol + myristate
-
 ?
3.1.1.75 p-nitrophenyl palmitate + H2O
-
 Thermobifida sp. BCC23166 4-nitrophenol + palmitate
-
 ?
3.1.1.75 p-nitrophenyl valerate + H2O
-
 Thermobifida sp. BCC23166 4-nitrophenol + pentanoate
-
 ?
3.1.1.75 poly-[(R)-3-hydroxybutyrate] + H2O degradation of poly-[(R)-3-hydroxybutyrate] (PHB) films Thermobifida sp. BCC23166 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PhaZ-Th
-
 Thermobifida sp. BCC23166
3.1.1.75 polyhydroxyalkanoate depolymerase
-
 Thermobifida sp. BCC23166

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 50 55 substrate p-nitrophenyl butyrate with optimal activity at 50-55°C and pH 7-8 Thermobifida sp. BCC23166

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.75 additional information
-
 no significant difference in catalytic activity and thermostability is observed for the deglycosylated PhaZ-Th compared to the glycosylated form Thermobifida sp. BCC23166

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 7 8 substrate p-nitrophenyl butyrate with optimal activity at 50-55°C and pH 7-8 Thermobifida sp. BCC23166