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Literature summary extracted from

  • Cao, B.; Geng, A.; Loh, K.C.
    Induction of ortho- and meta-cleavage pathways in Pseudomonas in biodegradation of high benzoate concentration: MS identification of catabolic enzymes (2008), Appl. Microbiol. Biotechnol., 81, 99-107.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 K+ included in assay medium Pseudomonas putida
1.13.11.2 K+ included in assay medium Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Pseudomonas putida benzoate degadation pathway cis,cis-muconate
-
 ?
1.13.11.2 catechol + O2 Pseudomonas putida benzoate degadation pathway 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Pseudomonas putida
-
-
-
1.13.11.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 cells are disrupted and centrifugates, protein supernatant is separated by 2D gel electrophoresis Pseudomonas putida
1.13.11.2 cells are disrupted and centrifugates, protein supernatant is separated by 2D gel electrophoresis Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.1 cell culture strain P8, ATCC 49451 Pseudomonas putida
-
1.13.11.2 cell culture strain P8, ATCC 49451 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 0.138
-
 Intracellular enzyme activity of C12D in crude lysate Pseudomonas putida
1.13.11.2 0.018
-
 Intracellular enzyme activity of C23D in crude lysate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 catechol + O2 benzoate degadation pathway Pseudomonas putida cis,cis-muconate
-
 ?
1.13.11.2 catechol + O2 benzoate degadation pathway Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C12D
-
 Pseudomonas putida
1.13.11.1 catechol 1,2-dioxygenase
-
 Pseudomonas putida
1.13.11.2 C23D
-
 Pseudomonas putida
1.13.11.2 catechol 2,3-dioxygenase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 24
-
 assay at Pseudomonas putida
1.13.11.2 24
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5
-
 assay at Pseudomonas putida
1.13.11.2 7.5
-
 assay at Pseudomonas putida