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Literature summary extracted from

  • Harada, H.; Yu, F.; Okamoto, S.; Kuzuyama, T.; Utsumi, R.; Misawa, N.
    Efficient synthesis of functional isoprenoids from acetoacetate through metabolic pathway-engineered Escherichia coli (2008), Appl. Microbiol. Biotechnol., 81, 915-925.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.104 expressed in Escherichia coli BL21 (DE3) and JM109 cells Zingiber zerumbet
6.2.1.16 DNA and amino acid sequence determination, expression in Escherichia coli JM109, the engineered strain utilizes acetoacetate and can synthesize carotenoids, e.g. alpha-humulene, effectively, overview Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.16 additional information expression of a Streptomyces mevalonate pathway gene cluster starting from HMG-CoA synthase and including isopentenyl diphosphate isomerase, idi, type 2 gene and the yeast idi type 1 and rat acetoacetate-CoA ligase genes in Escherichia coli, overview Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.16 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.16 ATP + acetoacetate + CoA Rattus norvegicus
-
 AMP + diphosphate + acetoacetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.104 Zingiber zerumbet
-
 shampoo ginger
-
6.2.1.16 Rattus norvegicus
-
 gene aacl
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.104 (2E,6E)-farnesyl diphosphate
-
 Zingiber zerumbet alpha-humulene + diphosphate
-
 ?
6.2.1.16 ATP + acetoacetate + CoA
-
 Rattus norvegicus AMP + diphosphate + acetoacetyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.104 alpha-humulene synthase
-
 Zingiber zerumbet
4.2.3.104 ZSS1
-
 Zingiber zerumbet
6.2.1.16 Aacl
-
 Rattus norvegicus
6.2.1.16 Acetoacetyl-CoA ligase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.16 ATP
-
 Rattus norvegicus

Expression

EC Number Organism Comment Expression
4.2.3.104 Zingiber zerumbet in the presence of 1 mg/ml lithium acetoacetate, a 13.6fold increase in alpha-humulene production occurs compared with the control up