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Literature summary extracted from

  • Sun, S.Y.; Zhang, X.; Zhou, Q.; Chen, J.C.; Chen, G.Q.
    Microbial production of cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate by genetically modified Pseudomonas putida (2008), Appl. Microbiol. Biotechnol., 80, 977-984.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.12.10 synthesis Synthesis of useful chiral chemical products: biotransformation of benzoate to cis-diols Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.10 The genes benABC encoding benzoate dioxygenase are cloned into plasmid pSYM01 which harboring a cis-diol dehydrogenase knockout and overexpressed in Pseudomona putida KTSY01. Pseudomonas putida

General Stability

EC Number General Stability Organism
1.14.12.10 Pseudomonas putida may be one of these bacteria that exhibits a strong tolerance to the toxicity of benzoate. Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.10 benzoate + NADH + H+ + O2 Pseudomonas putida
-
 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
 ?
1.14.12.10 benzoate + NADH + H+ + O2 Pseudomonas putida
-
 cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). ?
1.14.12.10 benzoate + NADH + H+ + O2 Pseudomonas putida KT 2240
-
 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
 ?
1.14.12.10 benzoate + NADH + H+ + O2 Pseudomonas putida KT 2240
-
 cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.10 Pseudomonas putida
-
 and strain KTSY01
-
1.14.12.10 Pseudomonas putida KT 2240
-
 and strain KTSY01
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.10 additional information
-
 The recombinant bacteria shows a BZDO activity of 1.2 mmol benzoate per gram cell dry weight, while Pseudomonas putida KTSY01 shows a BZDO activity of only 0.09 mmol. Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.10 benzoate + NADH + H+ + O2
-
 Pseudomonas putida (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
 ?
1.14.12.10 benzoate + NADH + H+ + O2
-
 Pseudomonas putida KT 2240 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
 ?
1.14.12.10 benzoate + NADH + H+ + O2
-
 Pseudomonas putida cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). ?
1.14.12.10 benzoate + NADH + H+ + O2
-
 Pseudomonas putida KT 2240 cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+ Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield). ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.10 benABC genes are organized in a single cluster and are under benzoate induced regulation. The benABC genes jointly encode benzoate 1,2-dioxygenase, which catalyzes the dihydroxylation of benzoate to produce 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid Pseudomonas putida
1.14.12.10 benzoate 1,2-dioxygenase
-
 Pseudomonas putida
1.14.12.10 benzoate 1,2-dioxygenase system BZDOS Pseudomonas putida
1.14.12.10 benzoate dioxygenase
-
 Pseudomonas putida
1.14.12.10 BZDO enzyme
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.10 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.10 7
-
 assay at Pseudomonas putida