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Literature summary extracted from
- Three trehalose synthetic pathways in the acarbose-producing Actinoplanes sp. SN223/29 and evidence for the TreY role in biosynthesis of component C (2008), Appl. Microbiol. Biotechnol., 80, 767-778.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.15 | expression in Escherichia coli | Actinoplanes sp. |
| 5.4.99.15 | expressed in Escherichia coli BL21(DE3)pLysS cells | Actinoplanes sp. |
| 5.4.99.16 | expressed in Escherichia coli BL21(DE3)pLysS cells | Actinoplanes sp. |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.1.15 | in phosphate or acetate buffers, the activity of TpS1 is decreased to 25% and 30%, respectively, of activity in Tris-HCl buffer | Actinoplanes sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.15 | EDTA | - |
Actinoplanes sp. |  |
| 2.4.1.15 | additional information | not inhibitory: 2-mercaptoethanol | Actinoplanes sp. |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.15 | Mg2+ | 95% stimulation | Actinoplanes sp. | |
| 2.4.1.15 | Mn2+ | 20% stimulation | Actinoplanes sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.15 | 51000 | - |
x * 51000, calculated | Actinoplanes sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.15 | Actinoplanes sp. | A8QX04 | - |
- |
| 2.4.1.15 | Actinoplanes sp. SN223/29 | A8QX04 | - |
- |
| 5.4.99.15 | Actinoplanes sp. | - |
- |
- |
| 5.4.99.15 | Actinoplanes sp. SN223/29 | - |
- |
- |
| 5.4.99.16 | Actinoplanes sp. | A8QX00 | - |
- |
| 5.4.99.16 | Actinoplanes sp. SN223/29 | A8QX00 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.15 | Ni-NTA column chromatography | Actinoplanes sp. |
| 5.4.99.16 | Ni-NTA column chromatography | Actinoplanes sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.15 | ADP-alpha-D-glucose + D-glucose 6-phosphate | 4% of the activity with UDP-glucose | Actinoplanes sp. | ADP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
| 2.4.1.15 | ADP-alpha-D-glucose + D-glucose 6-phosphate | 4% of the activity with UDP-glucose | Actinoplanes sp. SN223/29 | ADP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
| 2.4.1.15 | GDP-alpha-D-glucose + D-glucose 6-phosphate | 23% of the activity with UDP-glucose | Actinoplanes sp. | GDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
| 2.4.1.15 | GDP-alpha-D-glucose + D-glucose 6-phosphate | 23% of the activity with UDP-glucose | Actinoplanes sp. SN223/29 | GDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
| 2.4.1.15 | additional information | no substrate: CDP-glucose, fructose 6-phosphate | Actinoplanes sp. | ? | - |
? | |
| 2.4.1.15 | additional information | no substrate: CDP-glucose, fructose 6-phosphate | Actinoplanes sp. SN223/29 | ? | - |
? | |
| 2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Actinoplanes sp. | UDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
| 2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Actinoplanes sp. SN223/29 | UDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
| 5.4.99.15 | acarbose | - |
Actinoplanes sp. | ? | - |
? | |
| 5.4.99.15 | acarbose | - |
Actinoplanes sp. SN223/29 | ? | - |
? | |
| 5.4.99.15 | maltohexaose | - |
Actinoplanes sp. | alpha-maltotetraosyl trehalose | - |
? | |
| 5.4.99.15 | maltohexaose | - |
Actinoplanes sp. SN223/29 | alpha-maltotetraosyl trehalose | - |
? | |
| 5.4.99.16 | maltose | - |
Actinoplanes sp. | alpha,alpha-trehalose | - |
? | |
| 5.4.99.16 | maltose | - |
Actinoplanes sp. SN223/29 | alpha,alpha-trehalose | - |
? | |
| 5.4.99.16 | additional information | does not use acarbose and glucose as substrates | Actinoplanes sp. | ? | - |
? | |
| 5.4.99.16 | additional information | does not use acarbose and glucose as substrates | Actinoplanes sp. SN223/29 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.15 | ? | x * 51000, calculated | Actinoplanes sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | maltooligosyltrehalose synthase | - |
Actinoplanes sp. |
| 5.4.99.15 | TreY | - |
Actinoplanes sp. |
| 5.4.99.16 | Trehalose synthase | - |
Actinoplanes sp. |
| 5.4.99.16 | TreS | - |
Actinoplanes sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.15 | 35 | - |
- |
Actinoplanes sp. |
| 5.4.99.15 | 40 | - |
in 100 mM phosphate buffer | Actinoplanes sp. |
| 5.4.99.16 | 40 | - |
in 100 mM phosphate buffer | Actinoplanes sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.15 | 7.5 | - |
- |
Actinoplanes sp. |
| 5.4.99.15 | 7.5 | - |
in 100 mM phosphate buffer | Actinoplanes sp. |
| 5.4.99.16 | 7 | - |
in 100 mM phosphate buffer | Actinoplanes sp. |
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Release 2023.1 (January 2023)
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