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Literature summary extracted from

  • Li, Y.; Yu, H.; Cao, H.; Lau, K.; Muthana, S.; Tiwari, V.K.; Son, B.; Chen, X.
    Pasteurella multocida sialic acid aldolase: a promising biocatalyst (2008), Appl. Microbiol. Biotechnol., 79, 963-970.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.3
-
Escherichia coli K-12
4.1.3.3 into the vector pET22b+ for expression in Eschericha coli cells Pasteurella multocida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 2 3 pyruvate
-
Pasteurella multocida
4.1.3.3 2.5
-
N-acetylneuraminate
-
Escherichia coli K-12
4.1.3.3 4.9
-
N-acetylneuraminate
-
Pasteurella multocida
4.1.3.3 22
-
pyruvate
-
Escherichia coli K-12
4.1.3.3 180
-
N-acetyl-D-mannosamine
-
Escherichia coli K-12
4.1.3.3 220
-
N-acetyl-D-mannosamine
-
Pasteurella multocida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 33000
-
determined by SDS-PAGE Pasteurella multocida
4.1.3.3 33000
-
determined by SDS-PAGE Escherichia coli K-12
4.1.3.3 33400
-
calculated Escherichia coli K-12
4.1.3.3 33700
-
molecular mass Pasteurella multocida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.3 N-acetylneuraminate Pasteurella multocida
-
N-acetyl-D-mannosamine + pyruvate
-
r
4.1.3.3 N-acetylneuraminate Escherichia coli K-12
-
N-acetyl-D-mannosamine + pyruvate
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Escherichia coli K-12 P0A6L4
-
-
4.1.3.3 Pasteurella multocida Q9CKB0 the sequence of the cloned gene differs on six bases, T228C, T303C, C624T, G777A, C786T and C832T
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.3
-
Escherichia coli K-12
4.1.3.3 using a HisTrap_FF column Pasteurella multocida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 5-O-methyl-N-acetyl-D-mannosamine + pyruvate
-
Pasteurella multocida 8-O-methyl-N-acetylneuraminate
-
?
4.1.3.3 N-acetylneuraminate
-
Pasteurella multocida N-acetyl-D-mannosamine + pyruvate
-
r
4.1.3.3 N-acetylneuraminate
-
Escherichia coli K-12 N-acetyl-D-mannosamine + pyruvate
-
r

Synonyms

EC Number Synonyms Comment Organism
4.1.3.3 EcNanA
-
Escherichia coli K-12
4.1.3.3 N-Acetylneuraminate lyase
-
Pasteurella multocida
4.1.3.3 N-Acetylneuraminate lyase
-
Escherichia coli K-12
4.1.3.3 NanA
-
Pasteurella multocida
4.1.3.3 NanA
-
Escherichia coli K-12
4.1.3.3 PmNanA
-
Pasteurella multocida
4.1.3.3 Sialic acid aldolase
-
Pasteurella multocida
4.1.3.3 Sialic acid aldolase
-
Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.3 37
-
kinetic assay Pasteurella multocida
4.1.3.3 37
-
kinetic assay Escherichia coli K-12

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.3 1.8
-
pyruvate
-
Escherichia coli K-12
4.1.3.3 1.9
-
pyruvate
-
Pasteurella multocida
4.1.3.3 9
-
N-acetyl-D-mannosamine
-
Escherichia coli K-12
4.1.3.3 10
-
N-acetylneuraminate
-
Escherichia coli K-12
4.1.3.3 11
-
N-acetyl-D-mannosamine
-
Pasteurella multocida
4.1.3.3 16
-
N-acetylneuraminate
-
Pasteurella multocida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.3 7.5
-
kinetic assay Pasteurella multocida
4.1.3.3 7.5
-
kinetic assay Escherichia coli K-12

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.3 6 9
-
Pasteurella multocida
4.1.3.3 6 9
-
Escherichia coli K-12