EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.104 | Cyanate | positive regulation | Pseudomonas oleovorans | |
4.2.1.104 | cyanide | positive regulation | Pseudomonas oleovorans | |
4.2.1.104 | Urea | strong inducer of cyanase activity | Pseudomonas oleovorans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.104 | expression in Escherichia coli DH5 alpha | Pseudomonas oleovorans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.104 | additional information | mutant lacking the cynS gene, mutant is unable to use cyanate as the sole nitrogen source but shows the same resistance to cyanate as the wild-type strain, cynS-mutant is not affected in its ability to degrade cyanide, which unambiguously indicates that cyanate is not a central metabolite in cyanide assimilation | Pseudomonas oleovorans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.104 | ammonium | negative regulation even in the presence of cyanate. The negative effect exerted by ammonium on cyanate assimilation seems to take place at the level of gene expression, since the addition of ammonium to cells growing in cyanate had no effect on either cyanase activity or cyanate consumption | Pseudomonas oleovorans | |
4.2.1.104 | azide | - |
Pseudomonas oleovorans | |
4.2.1.104 | additional information | the enzyme is insensitive to 10 mM thiocyanate, a competitive inhibitor of some cyanases | Pseudomonas oleovorans | |
4.2.1.104 | nitrate | - |
Pseudomonas oleovorans | |
4.2.1.104 | p-hydroxymercuribenzoate | - |
Pseudomonas oleovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.104 | 2.4 | - |
CNO- | - |
Pseudomonas oleovorans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.104 | cytoplasm | - |
Pseudomonas oleovorans | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.104 | 16000 | - |
- |
Pseudomonas oleovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.104 | cyanate + ? | Pseudomonas oleovorans | - |
? | - |
? | |
4.2.1.104 | cyanate + ? | Pseudomonas oleovorans CECT 5344 | - |
? | - |
? | |
4.2.1.104 | additional information | Pseudomonas oleovorans | no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation | ? | - |
? | |
4.2.1.104 | additional information | Pseudomonas oleovorans CECT 5344 | no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.104 | Pseudomonas oleovorans | A4GXA0 | expression in Escherichia coli DH5 alpha | - |
4.2.1.104 | Pseudomonas oleovorans CECT 5344 | A4GXA0 | expression in Escherichia coli DH5 alpha | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.104 | precipitation, chromatography | Pseudomonas oleovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.104 | CNO- + HCO3- + 2 H+ | - |
Pseudomonas oleovorans | NH3 + 2 CO2 | - |
? | |
4.2.1.104 | CNO- + HCO3- + 2 H+ | - |
Pseudomonas oleovorans CECT 5344 | NH3 + 2 CO2 | - |
? | |
4.2.1.104 | cyanate + ? | - |
Pseudomonas oleovorans | ? | - |
? | |
4.2.1.104 | cyanate + ? | - |
Pseudomonas oleovorans CECT 5344 | ? | - |
? | |
4.2.1.104 | additional information | no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation | Pseudomonas oleovorans | ? | - |
? | |
4.2.1.104 | additional information | cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source | Pseudomonas oleovorans | ? | - |
? | |
4.2.1.104 | additional information | no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation | Pseudomonas oleovorans CECT 5344 | ? | - |
? | |
4.2.1.104 | additional information | cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source | Pseudomonas oleovorans CECT 5344 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.104 | cyanase | - |
Pseudomonas oleovorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.104 | 65 | - |
- |
Pseudomonas oleovorans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.104 | 30 | 70 | the thermostability range represents temperatures at which the enzyme retains more than 25% of its original activity after 30 min. Higher temperatures result in the irreversible inactivation of the enzyme | Pseudomonas oleovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.104 | 8.5 | - |
- |
Pseudomonas oleovorans |