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Literature summary extracted from

  • Luque-Almagro, V.M.; Huertas, M.J.; Saez, L.P.; Luque-Romero, M.M.; Moreno-Vivian, C.; Castillo, F.; Roldan, M.D.; Blasco, R.
    Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an enzyme that is not essential for cyanide assimilation (2008), Appl. Environ. Microbiol., 74, 6280-6288.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.104 Cyanate positive regulation Ectopseudomonas oleovorans
4.2.1.104 cyanide positive regulation Ectopseudomonas oleovorans
4.2.1.104 Urea strong inducer of cyanase activity Ectopseudomonas oleovorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.104 expression in Escherichia coli DH5 alpha Ectopseudomonas oleovorans

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.104 additional information mutant lacking the cynS gene, mutant is unable to use cyanate as the sole nitrogen source but shows the same resistance to cyanate as the wild-type strain, cynS-mutant is not affected in its ability to degrade cyanide, which unambiguously indicates that cyanate is not a central metabolite in cyanide assimilation Ectopseudomonas oleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.104 ammonium negative regulation even in the presence of cyanate. The negative effect exerted by ammonium on cyanate assimilation seems to take place at the level of gene expression, since the addition of ammonium to cells growing in cyanate had no effect on either cyanase activity or cyanate consumption Ectopseudomonas oleovorans
4.2.1.104 azide
-
 Ectopseudomonas oleovorans
4.2.1.104 additional information the enzyme is insensitive to 10 mM thiocyanate, a competitive inhibitor of some cyanases Ectopseudomonas oleovorans
4.2.1.104 nitrate
-
 Ectopseudomonas oleovorans
4.2.1.104 p-hydroxymercuribenzoate
-
 Ectopseudomonas oleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.104 2.4
-
 CNO-
-
 Ectopseudomonas oleovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.104 cytoplasm
-
 Ectopseudomonas oleovorans 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.104 16000
-
-
 Ectopseudomonas oleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.104 cyanate + ? Ectopseudomonas oleovorans
-
 ?
-
 ?
4.2.1.104 cyanate + ? Ectopseudomonas oleovorans CECT 5344
-
 ?
-
 ?
4.2.1.104 additional information Ectopseudomonas oleovorans no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation ?
-
 ?
4.2.1.104 additional information Ectopseudomonas oleovorans CECT 5344 no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.104 Ectopseudomonas oleovorans A4GXA0 expression in Escherichia coli DH5 alpha
-
4.2.1.104 Ectopseudomonas oleovorans CECT 5344 A4GXA0 expression in Escherichia coli DH5 alpha
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.104 precipitation, chromatography Ectopseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.104 CNO- + HCO3- + 2 H+
-
 Ectopseudomonas oleovorans NH3 + 2 CO2
-
 ?
4.2.1.104 CNO- + HCO3- + 2 H+
-
 Ectopseudomonas oleovorans CECT 5344 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + ?
-
 Ectopseudomonas oleovorans ?
-
 ?
4.2.1.104 cyanate + ?
-
 Ectopseudomonas oleovorans CECT 5344 ?
-
 ?
4.2.1.104 additional information no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation Ectopseudomonas oleovorans ?
-
 ?
4.2.1.104 additional information cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source Ectopseudomonas oleovorans ?
-
 ?
4.2.1.104 additional information no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation Ectopseudomonas oleovorans CECT 5344 ?
-
 ?
4.2.1.104 additional information cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source Ectopseudomonas oleovorans CECT 5344 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.104 cyanase
-
 Ectopseudomonas oleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.104 65
-
-
 Ectopseudomonas oleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.104 30 70 the thermostability range represents temperatures at which the enzyme retains more than 25% of its original activity after 30 min. Higher temperatures result in the irreversible inactivation of the enzyme Ectopseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.104 8.5
-
-
 Ectopseudomonas oleovorans