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Literature summary extracted from

  • Shinzato, N.; Enoki, M.; Sato, H.; Nakamura, K.; Matsui, T.; Kamagata, Y.
    Specific DNA binding of a potential transcriptional regulator, inosine 5-monophosphate dehydrogenase-related protein VII, to the promoter region of a methyl coenzyme M reductase I-encoding operon retrieved from Methanothermobacter thermautotrophicus strain DELTAH (2008), Appl. Environ. Microbiol., 74, 6239-6247.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.4.1 genes in operons mrt and mcr, DNA and amino acid sequence determination, promoter region, determination of operator regions of the mcr and mrt operons, and of cis elements and trans-acting factors responsible for the gene expression of MCRs by using electrophoretic mobility shift assay and affinity particle purification. IMP dehydrogenase-related protein VII, IMPDH VII encoded by MTH126, is a plausible candidate for the transcriptional regulator of the mcr operon in this methanogen, the binding site of IMPDH VII mostly overlaps the factor B-responsible element-TATA box of the mcr operon, overview. Expression in Escherichia coli strain BL21(DE3) Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter thermautotrophicus
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 a hydrogenotrophic methanogen, strain DELTAH DSM 1053, genes encoded in operons mrt and mcr
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Synonyms

EC Number Synonyms Comment Organism
2.8.4.1 MCR I
-
 Methanothermobacter thermautotrophicus
2.8.4.1 methyl coenzyme M reductase I
-
 Methanothermobacter thermautotrophicus