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Literature summary extracted from

  • Steinle, A.; Oppermann-Sanio, F.B.; Reichelt, R.; Steinbuechel, A.
    Synthesis and accumulation of cyanophycin in transgenic strains of Saccharomyces cerevisiae (2008), Appl. Environ. Microbiol., 74, 3410-3418.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue Synechocystis sp.
6.3.2.30 gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.29 Mg2+
-
 Synechocystis sp.
6.3.2.30 Mg2+
-
 Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Synechocystis sp.
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Synechocystis sp.
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 Synechocystis sp.
-
 strain PCC 6308, gene cphA6308
-
6.3.2.30 Synechocystis sp.
-
 strain PCC 6308, gene cphA6308
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Synechocystis sp. ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Synechocystis sp. ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 CphA
-
 Synechocystis sp.
6.3.2.29 cyanophycin synthetase
-
 Synechocystis sp.
6.3.2.29 More cf. EC 6.3.2.30 Synechocystis sp.
6.3.2.30 CphA
-
 Synechocystis sp.
6.3.2.30 cyanophycin synthetase
-
 Synechocystis sp.
6.3.2.30 More cf. EC 6.3.2.29 Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.29 ATP
-
 Synechocystis sp.
6.3.2.30 ATP
-
 Synechocystis sp.