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Literature summary extracted from

  • Kim, J.H.; Lim, B.C.; Yeom, S.J.; Kim, Y.S.; Kim, H.J.; Lee, J.K.; Lee, S.H.; Kim, S.W.; Oh, D.K.
    Differential selectivity of the Escherichia coli cell membrane shifts the equilibrium for the enzyme-catalyzed isomerization of galactose to tagatose (2008), Appl. Environ. Microbiol., 74, 2307-2313.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.3.1.4 synthesis an Escherichia coli galactose kinase gene knockout strain, which contains the L-arabinose isomerase gene to isomerize D-galactose to D-tagatose, shows a higher conversion yield of tagatose because galactose is not metabolized by endogenous galactose kinase. In whole cells of the galactose kinase knockout strain, the isomerase-catalyzed reaction exhibits an equilibrium shift towards tagatose, producing a tagatose fraction of 68% at 37°C, whereas the purified L-arabinose isomerase gives a tagatose equilibriumfraction of 36% Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 11
-
 D-tagatose pH 7.0, 37°C, purified enzyme Escherichia coli
5.3.1.4 114
-
 D-tagatose pH 7.0, 37°C, whole cells Escherichia coli
5.3.1.4 256
-
 D-galactose pH 7.0, 37°C, whole cells Escherichia coli
5.3.1.4 365
-
 D-galactose pH 7.0, 37°C, purified enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 Escherichia coli D-tagatose
-
 r
5.3.1.4 D-tagatose
-
 Escherichia coli D-galactose
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.4 0.06
-
 D-tagatose pH 7.0, 37°C, purified enzyme Escherichia coli
5.3.1.4 0.82
-
 D-tagatose pH 7.0, 37°C, whole cells Escherichia coli
5.3.1.4 1.02
-
 D-galactose pH 7.0, 37°C, purified enzyme Escherichia coli
5.3.1.4 4.21
-
 D-galactose pH 7.0, 37°C, whole cells Escherichia coli