Literature summary extracted from

Tang, H.; Wang, S.; Ma, L.; Meng, X.; Deng, Z.; Zhang, D.; Ma, C.; Xu, P.
A novel gene, encoding 6-hydroxy-3-succinoylpyridine hydroxylase, involved in nicotine degradation by Pseudomonas putida strain S16 (2008), Appl. Environ. Microbiol., 74, 1567-1574.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.13.163	expression in Escherichia coli	Pseudomonas putida

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.13.163	38000	-	x * 38000, SDS-PAGE and calculated	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.13.163	Pseudomonas putida	B1N1A2	-	-
1.14.13.163	Pseudomonas putida S16	B1N1A2	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.13.163	4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2	-	Pseudomonas putida	2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O	-	?
1.14.13.163	4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2	-	Pseudomonas putida S16	2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.13.163	?	x * 38000, SDS-PAGE and calculated	Pseudomonas putida

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.13.163	NIC	-	Pseudomonas putida

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.14.13.163	37	-	assay at	Pseudomonas putida

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.14.13.163	7	-	assay at	Pseudomonas putida

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.13.163	NADH	required	Pseudomonas putida

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Release 2023.1 (January 2023)