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Literature summary extracted from

  • Mutanda, T.; Wilhelmi, B.; Whiteley, C.G.
    Controlled production of fructose by an exoinulinase from Aspergillus ficuum (2009), Appl. Biochem. Biotechnol., 159, 65-77.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.80 food industry diabetics Aspergillus ficuum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 D-glucose competitive inhibitor Aspergillus ficuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.80 4.75
-
 inulin incubation at 50°C, 1 h Aspergillus ficuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 63000
-
 SDS-PAGE Aspergillus ficuum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Aspergillus ficuum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 4.2-fold purification, 21% yield Aspergillus ficuum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.80 12300
-
 pH 5.0, 50°C Aspergillus ficuum

Storage Stability

EC Number Storage Stability Organism
3.2.1.80 50°C, pH 5.4, stable for 60 min without decrease in activity, after 12 h only 96% residual activity, after 72 h only 63.8% residual activity Aspergillus ficuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + H2O
-
 Aspergillus ficuum D-fructose + ? after incubation at 50°C for 1 h ?
3.2.1.80 inulin + H2O
-
 Aspergillus ficuum 1-kestose + ?
-
 ?
3.2.1.80 inulin + H2O
-
 Aspergillus ficuum beta-D-Fruf-(2-1)-beta-D-Fruf + ?
-
 ?
3.2.1.80 inulin + H2O
-
 Aspergillus ficuum sucrose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 exoinulinase
-
 Aspergillus ficuum
3.2.1.80 fructofuranosyl hydrolase
-
 Aspergillus ficuum
3.2.1.80 glycoside hydrolase family 32 Aspergillus ficuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 50
-
-
 Aspergillus ficuum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.80 30 50
-
 Aspergillus ficuum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.80 50
-
 stable for 60 min without decrease in activity, stable for 12 h with 96% residual activity, stable for 72 h with 63.8% residual activity Aspergillus ficuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5.4
-
-
 Aspergillus ficuum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.80 5 6.2
-
 Aspergillus ficuum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.80 5.4
-
 stable for 60 min without decrease in activity, stable for 12 h with 96% residual activity, stable for 72 h with 63.8% residual activity Aspergillus ficuum