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Literature summary extracted from

  • Dutta, S.; Ray, L.
    Production and characterization of an alkaline thermostable crude lipase from an isolated strain of Bacillus cereus C(7) (2009), Appl. Biochem. Biotechnol., 159, 142-154.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 sodium deoxycholate 23% increase in activity at 0.5% (w/v) Bacillus cereus
3.1.1.3 sodium taurocholate 20% increase in activity at 0.5% (w/v) Bacillus cereus

General Stability

EC Number General Stability Organism
3.1.1.3 the enzyme remains stable in the presence of trypsin Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 benzalkonium chloride little inhibitory effect Bacillus cereus
3.1.1.3 Brij 35 little inhibitory effect Bacillus cereus
3.1.1.3 cetyltrimethylammonium bromide
-
 Bacillus cereus
3.1.1.3 cholic acid 90% residual activity at 0.5% (w/v) Bacillus cereus
3.1.1.3 Co2+ 38.83% residual activity at 5 mM Bacillus cereus
3.1.1.3 Fe3+ complete inhibition at 5 mM Bacillus cereus
3.1.1.3 Mn2+ complete inhibition at 5 mM Bacillus cereus
3.1.1.3 Polyethylene glycol little inhibitory effect Bacillus cereus
3.1.1.3 Sn2+ complete inhibition at 5 mM Bacillus cereus
3.1.1.3 Span 40 little inhibitory effect Bacillus cereus
3.1.1.3 Triton X-100
-
 Bacillus cereus
3.1.1.3 Tween 20 little inhibitory effect Bacillus cereus
3.1.1.3 Tween 80 little inhibitory effect Bacillus cereus
3.1.1.3 Zn2+ 72.41% residual activity at 5 mM Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ 104% increase in activity in the presence of 1 mM Ca2+ Bacillus cereus
3.1.1.3 Co2+ 112.56% increase in activity in the presence of 0.1 mM Co2+, the stimulatory effect is not observed at higher concentrations Bacillus cereus
3.1.1.3 Cu2+ 83.03% increase in activity in the presence of 1 mM Cu2+ Bacillus cereus
3.1.1.3 Mg2+ 46.66% increase in activity in the presence of 0.1 mM Mg2+, the stimulatory effect is not observed at higher concentrations Bacillus cereus
3.1.1.3 Zn2+ 41.5% increase in activity in the presence of 1 mM Zn2+, the stimulatory effect is not observed at higher concentrations Bacillus cereus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 additional information commercial detergents enhance the enzyme activity, the enzyme retains 60% or more activity in presence of commercial detergents Bacillus cereus
3.1.1.3 SDS SDS has little stimulatory effect Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Bacillus cereus
-
-
-
3.1.1.3 Bacillus cereus C(7)
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.1.1.3 the enzyme is stable in the presence of oxidizing agents like 1% (v/v) hydrogen peroxide and 1% (w/v) sodium hypochlorite Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 olive oil + H2O
-
 Bacillus cereus ?
-
 ?
3.1.1.3 olive oil + H2O
-
 Bacillus cereus C(7) ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 60
-
-
 Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30 70 lipase activity increases progressively with increase in temperature and maximum activity is found to be at 60°C, then the activity decreases with further increase in temperature Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 7.6 9 at 60°C, using Tris-HCl buffer Bacillus cereus
3.1.1.3 9 10 at 60°C, using glycine-NaOH buffer Bacillus cereus