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Literature summary extracted from

  • Kudan, S.; Pichyangkura, R.
    Purification and characterization of thermostable chitinase from Bacillus licheniformis SK-1 (2009), Appl. Biochem. Biotechnol., 157, 23-35.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information Chi72 follows the classical Michaelis-Menten kinetics Bacillus licheniformis
3.2.1.14 0.23
-
 colloidal chitin
-
 Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 72000
-
 x * 72000, Chi72, SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus licheniformis
-
 isolated from soil in Angthong Province, Thailand
-
3.2.1.14 Bacillus licheniformis SK-1
-
 isolated from soil in Angthong Province, Thailand
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme 11.5fold from strain SK-1 by colloidal chitin affinity and anion exchange chromatography Bacillus licheniformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 2 2.5 purified enzyme Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal , regenerated, or partially N-acetylated chitin substrates Bacillus licheniformis ?
-
 ?
3.2.1.14 chitin + H2O colloidal , regenerated, or partially N-acetylated chitin substrates Bacillus licheniformis SK-1 ?
-
 ?
3.2.1.14 chitosan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.14 chitosan + H2O
-
 Bacillus licheniformis SK-1 ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Bacillus licheniformis SK-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 72000, Chi72, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chi72
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 55
-
 Chi72 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 40 70 active range, Chi72 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40
-
 4 h, purified Chi72, stable Bacillus licheniformis
3.2.1.14 50
-
 2 h, purified Chi72, stable Bacillus licheniformis
3.2.1.14 60
-
 half-life 90 min, purified Chi72 Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
 maximum 1 of Chi72 Bacillus licheniformis
3.2.1.14 8
-
 maximum 2 of Chi72 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 6 9
-
 Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 4 9 24 h, purified Chi72, stable Bacillus licheniformis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Bacillus licheniformis Chi72
-
 4.62