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Literature summary extracted from

  • Gaur, R.; Gupta, G.N.; Vamsikrishnan, M.; Khare, S.K.
    Protein-coated microcrystals of Pseudomonas aeruginosa PseA lipase (2008), Appl. Biochem. Biotechnol., 151, 160-166.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.3 synthesis lipases constitute one of the most important groups of biocatalysts due to their ability to catalyze three different kinds of reactions viz. hydrolysis, esterification, and transesterification with unique chemo-, regio-, and enantiospecific selectivity. Lipase-based processes are employed for oil/fat processing, synthesis of industrially important oleochemicals, enantiopure pharmaceuticals, agrochemicals, flavor esters, structured lipids, and biodiesel production Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 additional information preparation of highly active lipase protein-coated microcrystals, PAL PCMC, by immobilization of protein onto K2SO4 as excipient solid support carrier and n-propanol as precipitating solvent, the Vmax, Km, and temperature optimum for PAL PCMC are increased compared to the soluble enzyme, PAL PCMC exhibits an 11.5fold increase in rate of hydrolysis with 4-nitrophenyl palmitate and 200fold with 1-phenylethanol compared to free enzyme, also the immobilized enzyme is thermally more stable with 4.65, 2.56, and 1.24fold improvement in half-lives at 45°C, 55°C, and 60°C compared to free PseA lipase, overview Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 589
-
 4-nitrophenyl palmitate free enzyme Pseudomonas aeruginosa
3.1.1.3 679.8
-
 4-nitrophenyl palmitate immobilied enzyme Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 triacylglycerol + H2O Pseudomonas aeruginosa
-
 diacylglycerol + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O the immobilized enzyme PAL PCMC works as better catalyst for hydrolysis of pNPP in n-heptane medium Pseudomonas aeruginosa palmitate + 4-nitrophenol
-
 ?
3.1.1.3 butyl butyrate + H2O
-
 Pseudomonas aeruginosa butane + butyrate
-
 ?
3.1.1.3 ethyl butyrate + H2O
-
 Pseudomonas aeruginosa ethane + butyrate
-
 ?
3.1.1.3 triacylglycerol + H2O
-
 Pseudomonas aeruginosa diacylglycerol + a carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Pseudomonas aeruginosa
3.1.1.3 PseA
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
 free enzyme Pseudomonas aeruginosa
3.1.1.3 45
-
 immobilied enzyme Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 25 50
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 additional information
-
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5.5 9
-
 Pseudomonas aeruginosa