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Literature summary extracted from

  • Sugimoto, A.; Shiraki, M.; Hatakeyama, S.; Saito, T.
    Secretion pathway for the poly(3-hydroxybutyrate) depolymerase in Ralstonia pickettii T1 (2008), Antonie van Leeuwenhoek, 94, 223-232.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 expressed in Escherichia coli Ralstonia pickettii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.75 additional information N17-mutant unable to degrade poly(3-hydroxybutyrate), obtained with the random insertion of a minitransposon, Tn5. Mutant accumulates the poly(3-hydroxybutyrate) depolymerase in the periplasm and cytoplasm, and does not secrete the enzyme into the external medium Ralstonia pickettii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Ralstonia pickettii
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Ralstonia pickettii P12625 T1
-
3.1.1.75 Ralstonia pickettii T1 P12625 T1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75
-
 Ralstonia pickettii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly(3-hydroxybutyrate) + H2O
-
 Ralstonia pickettii ?
-
 ?
3.1.1.75 poly(3-hydroxybutyrate) + H2O
-
 Ralstonia pickettii T1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 poly(3-hydroxybutyrate) depolymerase
-
 Ralstonia pickettii