Literature summary extracted from
Shibuya, N.; Tanaka, M.; Yoshida, M.; Ogasawara, Y.; Togawa, T.; Ishii, K.; Kimura, H.
3-Mercaptopyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain (2009), Antioxid. Redox Signal., 11, 703-714.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.8.1.2 |
transient transfection of human embryonic kidney HEK 293-F cells |
Mus musculus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.8.1.2 |
C247S |
site-directed mutagenesis, transient transfection of human embryonic kidney HEK 293-F cells, mutant completely lose the activity to metabolize 3-mercaptopyruvate that is assessed by measuring its products either pyruvate or H2S, the levels of bound sulfane sulfur are not increased |
Mus musculus |
2.8.1.2 |
R187G |
site-directed mutagenesis, transient transfection of human embryonic kidney HEK 293-F cells, mutant, which greatly lose the activity, decreases the levels of bound sulfane sulfur, but not statistically significant, no H2S production |
Mus musculus |
2.8.1.2 |
R196G |
site-directed mutagenesis, transient transfection of human embryonic kidney HEK 293-F cells, mutant, which partially lose the activity, does not decrease the levels of bound sulfane sulfur, no H2S production |
Mus musculus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.8.1.2 |
3-mercaptopyruvate + cyanide |
Mus musculus |
H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT) |
H2S + ? |
a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase |
? |
|
2.8.1.2 |
additional information |
Mus musculus |
3-mercaptopyruvate sulfurtransferase produces bound sulfane sulfur |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.8.1.2 |
Mus musculus |
Q99J99 |
expression in human embryonic kidney HEK 293-F cells |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.8.1.2 |
3-mercaptopyruvate + cyanide |
H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT) |
Mus musculus |
H2S + ? |
a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase |
? |
|
2.8.1.2 |
additional information |
3-mercaptopyruvate sulfurtransferase produces bound sulfane sulfur |
Mus musculus |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.8.1.2 |
3-mercaptopyruvate sulfurtransferase |
- |
Mus musculus |
2.8.1.2 |
3MST |
- |
Mus musculus |