BRENDA - Enzyme Database show

Deoxyribonucleoside kinases activate nucleoside antibiotics in severely pathogenic bacteria

Sandrini, M.P.; Shannon, O.; Clausen, A.R.; Bjoerck, L.; Piskur, J.; Antimicrob. Agents Chemother. 51, 2726-2732 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.76
-
Staphylococcus aureus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.0292
-
2'-deoxyadenosine
pH 8.0
Staphylococcus aureus
2.7.1.76
0.0344
-
2'-deoxycytidine
pH 8.0
Staphylococcus aureus
2.7.1.76
0.192
-
2',2'-difluorodesoxycytidine
pH 8.0
Staphylococcus aureus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
26400
-
x * 26400, calculated
Staphylococcus aureus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Staphylococcus aureus
Q0H0H0
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + 2',2'-difluorodeoxycytidine
-
690459
Staphylococcus aureus
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxyadenosine
-
690459
Staphylococcus aureus
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxycytidine
-
690459
Staphylococcus aureus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.76
?
x * 26400, calculated
Staphylococcus aureus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.19
-
2',2'-difluorodeoxycytidine
pH 8.0
Staphylococcus aureus
2.7.1.76
0.56
-
2'-deoxycytidine
pH 8.0
Staphylococcus aureus
2.7.1.76
1.82
-
2'-deoxyadenosine
pH 8.0
Staphylococcus aureus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
-
Staphylococcus aureus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.0292
-
2'-deoxyadenosine
pH 8.0
Staphylococcus aureus
2.7.1.76
0.0344
-
2'-deoxycytidine
pH 8.0
Staphylococcus aureus
2.7.1.76
0.192
-
2',2'-difluorodesoxycytidine
pH 8.0
Staphylococcus aureus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
26400
-
x * 26400, calculated
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + 2',2'-difluorodeoxycytidine
-
690459
Staphylococcus aureus
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxyadenosine
-
690459
Staphylococcus aureus
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxycytidine
-
690459
Staphylococcus aureus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.76
?
x * 26400, calculated
Staphylococcus aureus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.19
-
2',2'-difluorodeoxycytidine
pH 8.0
Staphylococcus aureus
2.7.1.76
0.56
-
2'-deoxycytidine
pH 8.0
Staphylococcus aureus
2.7.1.76
1.82
-
2'-deoxyadenosine
pH 8.0
Staphylococcus aureus