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Literature summary extracted from
- The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite green (2009), Anal. Chim. Acta, 636, 105-110.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.53 | EDTA | EDTA at 0.10 mM slightly activates PDE4 | Homo sapiens |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.53 | expressed in Escherichia coli strain BL21 | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.53 | additional information | not affected by Ca2+, Fe2+, Mn2+, HSCH2CH2OH, methanol, and dimethylsulfoxide | Homo sapiens | |
| 3.1.4.53 | rolipram | - |
Homo sapiens | |
| 3.1.4.53 | Zn2+ | more than 90% inhibition at 0.05 mM Zn2+ in the presence of 0.1 mM EDTA, inhibition can be greatly relieved with EDTA at 0.30 mM | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.53 | 0.0088 | - |
3',5'-cAMP | - |
Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.53 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.53 | ammonium sulfate fractionation and amylose-resin column chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.53 | 3',5'-cAMP + H2O | - |
Homo sapiens | 5'-AMP | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.53 | cyclic nucleotide phosphodiesterase 4 | - |
Homo sapiens |
| 3.1.4.53 | PDE4 | - |
Homo sapiens |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.53 | 0.00001 | - |
in 20 mM Tris-HCl at pH 7.4, 10 mM MgCl2, and 0.1 mM EDTA, at 30°C | Homo sapiens | rolipram | |
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