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Literature summary extracted from

  • Kanamori-Kataoka, M.; Seto, Y.
    Paraoxonase activity against nerve gases measured by capillary electrophoresis and characterization of human serum paraoxonase (PON1) polymorphism in the coding region (Q192R) (2009), Anal. Biochem., 385, 94-100.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.2 Q192R the mutant shows lower Vmax and kcat/Km values for soman and sarin than the wild type enzyme Homo sapiens
3.1.8.1 Q192R the mutant shows lower Vmax and kcat/Km values for soman and sarin than the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.86
-
 phenyl acetate wild type enzyme, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 8.0), at 25°C Homo sapiens
3.1.1.2 0.89
-
 phenyl acetate mutant enzyme Q192R, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 8.0), at 25°C Homo sapiens
3.1.8.1 0.074
-
 sarin mutant enzyme Q192R, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
3.1.8.1 0.2
-
 O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate mutant enzyme Q192R, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
3.1.8.1 0.2
-
 O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate wild type enzyme, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
3.1.8.1 0.21
-
 sarin wild type enzyme, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
3.1.8.1 0.27
-
 soman wild type enzyme, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
3.1.8.1 0.44
-
 soman mutant enzyme Q192R, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
3.1.8.1 0.94
-
 paraoxon wild type enzyme, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
3.1.8.1 1.2
-
 paraoxon mutant enzyme Q192R, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.2 Ca2+ required for activity Homo sapiens
3.1.8.1 Ca2+ required for activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 45000
-
 SDS-PAGE Homo sapiens
3.1.8.1 45000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens P27169
-
-
3.1.8.1 Homo sapiens P27169
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.2 glycoprotein
-
 Homo sapiens
3.1.8.1 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 blue agarose affinity chromatography and HiTrap DEAE column chromatography Homo sapiens
3.1.8.1 blue agarose affinity chromatography and HiTrap DEAE column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 serum
-
 Homo sapiens
-
3.1.8.1 serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.1 0.0012
-
 wild type enzyme from crude extract, using paraoxon as substrate Homo sapiens
3.1.8.1 0.0013
-
 mutant enzyme Q192R from crude extract, using paraoxon as substrate Homo sapiens
3.1.8.1 0.94
-
 mutant enzyme Q192R after 1018fold purification, using paraoxon as substrate Homo sapiens
3.1.8.1 1.21
-
 wild type enzyme after 1018fold purification, using paraoxon as substrate Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.1.2 4°C, purified PON1 in DEAE buffer, at least two weeks, remains stable Homo sapiens
3.1.8.1 4°C, purified PON1 in DEAE buffer, at least two weeks, remains stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 2-naphthyl acetate + H2O
-
 Homo sapiens 2-naphthol + acetate
-
 ?
3.1.1.2 phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.8.1 chlorpyrifos + H2O
-
 Homo sapiens ?
-
 ?
3.1.8.1 chlortion + H2O chlorthion is O,O-dimethyl O-(3-chloro-4-nitrophenyl) thionophosphate Homo sapiens ?
-
 ?
3.1.8.1 O-ethyl O-4-nitrophenyl phenylphosphonothioate + H2O
-
 Homo sapiens ?
-
 ?
3.1.8.1 O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate + H2O
-
 Homo sapiens ?
-
 ?
3.1.8.1 paraoxon + H2O
-
 Homo sapiens diethylphosphate + 4-nitrophenol
-
 ?
3.1.8.1 sarin + H2O PON1 hydrolyzes sarin more effectively than paraoxon, sarin is o-isopropyl methylphosphonofluoridate Homo sapiens methyl-phosphonic acid monofluoride + isopropyl alcohol
-
 ?
3.1.8.1 soman + H2O PON1 hydrolyzes soman more effectively than paraoxon, soman is o-pinacolyl methylphosphonofluoridate Homo sapiens methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 ? x * 45000, SDS-PAGE Homo sapiens
3.1.8.1 ? x * 45000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 paraoxonase
-
 Homo sapiens
3.1.8.1 PON1 the calcium-dependent glycoprotein is associated with high-density lipoprotein Homo sapiens