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Literature summary extracted from

  • Zhang, Z.; Park, Y.; Kemp, M.M.; Zhao, W.; Im, A.R.; Shaya, D.; Cygler, M.; Kim, Y.S.; Linhardt, R.J.
    Liquid chromatography-mass spectrometry to study chondroitin lyase action pattern (2009), Anal. Biochem., 385, 57-64.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.21 expressed in Escherichia coli Bacteroides thetaiotaomicron

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Paenarthrobacter aurescens
-
-
-
4.2.2.5 Pedobacter heparinus
-
-
-
4.2.2.20 Pedobacter heparinus
-
-
-
4.2.2.20 Proteus vulgaris
-
-
-
4.2.2.21 Bacteroides thetaiotaomicron Q8A2I1
-
-
4.2.2.21 Paenarthrobacter aurescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.5 chondroitin 4-sulfate exolytic action pattern Paenarthrobacter aurescens ? the products afforded by chondroitinase ACII contain primarily unsaturated disaccharide ?
4.2.2.5 chondroitin 4-sulfate random endolytic action pattern Pedobacter heparinus ? the products of chondroitinase AC contain unsaturated oligosaccharides of sizes ranging from disaccharide to decasaccharide ?
4.2.2.5 chondroitin 6-sulfate exolytic action pattern Paenarthrobacter aurescens ? the products afforded by chondroitinase ACII contain primarily unsaturated disaccharide ?
4.2.2.5 chondroitin 6-sulfate random endolytic action pattern Pedobacter heparinus ? the products of chondroitinase AC contain unsaturated oligosaccharides of sizes ranging from disaccharide to decasaccharide ?
4.2.2.20 chondroitin 4-sulfate liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products of chondroitinase ABC from Proteus vulgaris and chondroitinase AC from Flavobacterium heparinum contain unsaturated oligosaccharides of sizes ranging from disaccharide to decasaccharide, demonstrating that both are endolytic enzymes Proteus vulgaris ?
-
 ?
4.2.2.20 chondroitin 4-sulfate liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products of chondroitinase ABC from Proteus vulgaris and chondroitinase AC from Flavobacterium heparinum contain unsaturated oligosaccharides of sizes ranging from disaccharide to decasaccharide, demonstrating that both are endolytic enzymes Pedobacter heparinus ?
-
 ?
4.2.2.21 chondroitin 4-sulfate liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products afforded by chondroitinase ABC from Bacteroides thetaiotaomicron and chondroitinase ACII from Arthrobacter aurescens, contain primarily unsatuated disaccharide. These two exolytic enzymes showed different minor products, suggesting some subtle specificity differences between the actions of these two exolytic lyases on chondroitin sulfate A Paenarthrobacter aurescens ?
-
 ?
4.2.2.21 chondroitin 4-sulfate liquid chromatography-mass spectrometry is applied to determine the action pattern of different chondroitin lyases: products afforded by chondroitinase ABC from Bacteroides thetaiotaomicron and chondroitinase ACII from Arthrobacter aurescens, contain primarily unsatuated disaccharide. These two exolytic enzymes showed different minor products, suggesting some subtle specificity differences between the actions of these two exolytic lyases on chondroitin sulfate A Bacteroides thetaiotaomicron ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.5 chondroitinase AC
-
 Pedobacter heparinus
4.2.2.5 chondroitinase ACII
-
 Paenarthrobacter aurescens
4.2.2.20 chondroitinase ABC
-
 Proteus vulgaris
4.2.2.20 chondroitinase AC
-
 Pedobacter heparinus
4.2.2.21 chondroitinase ABC
-
 Bacteroides thetaiotaomicron
4.2.2.21 chondroitinase ACII
-
 Paenarthrobacter aurescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.20 37
-
 assay at Pedobacter heparinus
4.2.2.21 37
-
 assay at Paenarthrobacter aurescens
4.2.2.21 37
-
 assay at Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.20 7
-
 assay at Pedobacter heparinus
4.2.2.21 7
-
 assay at Paenarthrobacter aurescens
4.2.2.21 7
-
 assay at Bacteroides thetaiotaomicron