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Literature summary extracted from

  • Cailleteau, C.; Liagre, B.; Battu, S.; Jayat-Vignoles, C.; Beneytout, J.L.
    Increased cyclooxygenase-2 and thromboxane synthase expression is implicated in diosgenin-induced megakaryocytic differentiation in human erythroleukemia cells (2008), Anal. Biochem., 380, 26-34.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.5 diosgenin 10 microM increases cyclooxygenase-2 and thromboxane synthase expression and activities in HEL cells, 3.5fold at 96 h and 4.5fold at 144 h Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.99.5 BM 567 i.e. 2-(cyclohexylamino)-5-nitro-N-[(pentylamino)carbonyl]-benzenesulfonamide, 10 microM, a specific inhibitor for thromboxane synthase, decrease the acitivity 2.1fold before diosgenin treatment Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.5 additional information Homo sapiens cyclooxygenase-2 and thromboxane synthase are involved in the diosgenin-induced megakaryocytic differentiation in HEL cells ? for analysis of the thromboxane synthase activity the secretion of thromoxane B2 is measured. Thromboxane B2 is a nonenzymatic conversion product of thromobxane A1, the product of the thromboxane synthase activity ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.5 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.99.5 erythroleukemia cell
-
 Homo sapiens
-
5.3.99.5 HEL cell human erythroleukemia cells Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.5 additional information cyclooxygenase-2 and thromboxane synthase are involved in the diosgenin-induced megakaryocytic differentiation in HEL cells Homo sapiens ? for analysis of the thromboxane synthase activity the secretion of thromoxane B2 is measured. Thromboxane B2 is a nonenzymatic conversion product of thromobxane A1, the product of the thromboxane synthase activity ?
5.3.99.5 prostaglandin E2
-
 Homo sapiens thromboxane A2 + ? thromoboxane A2 is non-enzymatically converted to thromboxane B2, which is secretion is measured to analyze the enzyme activity ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.5 thromboxane synthase
-
 Homo sapiens
5.3.99.5 TXS
-
 Homo sapiens