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Literature summary extracted from

  • Ng, B.; Polyak, S.W.; Bird, D.; Bailey, L.; Wallace, J.C.; Booker, G.W.
    Escherichia coli biotin protein ligase: characterization and development of a high-throughput assay (2008), Anal. Biochem., 376, 131-136.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.15 diphosphate endproduct inhibition Escherichia coli
6.3.4.15 MgAMP endproduct inhibition, mechanism of inhibition, overview Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.15 additional information
-
 additional information kinetic analysis Escherichia coli
6.3.4.15 0.00145
-
 biotin pH 8.0, 37°C Escherichia coli
6.3.4.15 0.25
-
 ATP pH 8.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.15 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Escherichia coli biotin protein ligase, BPL required to complete acetyl-CoA carbox­ylase's capability for fatty acid biosynthesis AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Escherichia coli
-
 gene birA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Escherichia coli AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] biotin protein ligase, BPL required to complete acetyl-CoA carbox­ylase's capability for fatty acid biosynthesis Escherichia coli AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + fluorescein-labeled peptide 85-11 peptide 85-11 has the sequence MAGGLNDIFEAQKIEWHE with the lysine residue being targeted for biotinylation, development of a fluorescence polarization technology-based assay method, overview Escherichia coli ADP + diphosphate + biotinyl-fluorescein-labeled peptide 85-11
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin protein ligase
-
 Escherichia coli
6.3.4.15 BirA
-
 Escherichia coli
6.3.4.15 BPL
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.15 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.15 8
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.4.15 8.24
-
 pH 8.0, 37°C Escherichia coli MgAMP