Literature summary extracted from
Au, K.; Ren, J.; Walter, T.S.; Harlos, K.; Nettleship, J.E.; Owens, R.J.; Stuart, D.I.; Esnouf, R.M.
Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P) (2008), Acta Crystallogr. Sect. F, F64, 327-333.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.1.1.1 |
amplified by PCR, cloned and overexpressed with pOPINB in Escherichia coli Rosetta pLysS cells, His-tagged product |
Bacillus anthracis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.1.1.1 |
enzyme is subjected to a reductive-methylation procedure |
Bacillus anthracis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.1.1.1 |
(R)-1-aminoethylphosphonic acid |
in combination with pyridoxal 5'-phosphate |
Bacillus anthracis |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
5.1.1.1 |
exosporium |
- |
Bacillus anthracis |
43592 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.1.1.1 |
43810 |
- |
mass-spectrometry |
Bacillus anthracis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.1.1.1 |
L-alanine |
Bacillus anthracis |
- |
D-alanine |
enzyme provides D-Ala as a required compound for the synthesis of the peptidoglycan layer of the bacterial cell wall, Tolypocladium niveum requires alanine racemase for cyclosporin biosynthesis |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.1.1.1 |
Bacillus anthracis |
Q81VF6 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.1.1.1 |
L-alanine |
- |
Bacillus anthracis |
D-alanine |
enzyme provides D-Ala as a required compound for the synthesis of the peptidoglycan layer of the bacterial cell wall, Tolypocladium niveum requires alanine racemase for cyclosporin biosynthesis |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.1.1.1 |
dimer |
crystallography |
Bacillus anthracis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.1.1.1 |
BA0252 |
- |
Bacillus anthracis |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
5.1.1.1 |
pyridoxal 5'-phosphate |
C-terminal region of 1 subdomain: Arg138 donates a hydrogen bond to the phenolic O atom of PLP, Arg224 donates a hydrogen bond to the pyridinyl N atom of PLP, Lys41 forms an aldimine linkage with the PLP, eliminating water to form the Schiff base, C-terminal atoms of second subunit Ser209, Gly226 and Ile227 stabilize the PLP phosphate with the help of Ser209 O(gamma), Tyr45 O(eta) and Tyr359 O(eta) |
Bacillus anthracis |
|