Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yap, T.L.; Chen, Y.L.; Xu, T.; Wen, D.; Vasudevan, S.G.; Lescar, J.
    A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution (2007), Acta Crystallogr. Sect. F, F63, 78-83.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 expressed in Escherichia coli coli BL21 (DE3) cells Dengue virus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.48 hanging drop vapour diffusion method, with 0.1 M Tris-HCl pH 8.5, 0.8 M potassium/sodium tartrate, and 0.5% (w/v) PEG monomethyl ether 5000 Dengue virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Dengue virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 HisTrap HP column chromatography, Source 15S column chromatography, and gel filtration Dengue virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Dengue virus diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 DENV 3 polymerase
-
 Dengue virus
2.7.7.48 DENV RdRp
-
 Dengue virus
2.7.7.48 NS5
-
 Dengue virus
2.7.7.48 RNA-dependent RNA polymerase
-
 Dengue virus