EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.1.7 | cloning and overexpression of three different versions (eut-LpET151 his-tagged version (appends a cleavable 33-amino acid tag sequence to the N-terminus), Eut-L_NHIS (short His6-tagged version) and Eut-L_CHIS (short His6-tagged version)) of the protein is carried out directly from the Escherichia coli genome by selective PCR amplification. Selenomethione-derivatized proteins are obtained by growing cloned bacteria in selenomethionine-containing M9 minimal media. Protein overexpression and purification are performed. | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.1.7 | For crystallization trials, protein sample is dialyzed against 50 mM HEPES with pH 7.0 and concentrates to a final concentration of about 1 mg/ml. Purification results in highly pure protein that crystallizes readily under many different conditions, protein forms thin hexagonal plate-shaped crystals belonging to space group P3. Best crystals of Eut-L_NHIS are obtained in 3.3 M ammonium acetate, 5% polyethylene glycol 400 and 50 mM Tris buffer pH 7.5, crystals grow as hexagonal plates. Eut-L_CHIS crystals grow as single hexagonal plates with sharp edges. Crystals grow in 2 M NaCl, 100 mM phosphate, 100 mM MES buffer pH 6.5 and 4% PEG 400. | Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
4.3.1.7 | unusually high stability against different solvent conditions with respect to changes in pH and ionic strength. | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.3.1.7 | additional information | microcompartiment shell protein | Escherichia coli | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.1.7 | 66800 | - |
for the trimer, determination by gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.7 | ethanolamine | Escherichia coli | Escherichia coli has evolved specialized organelles (microcompartments) for the degradation of small molecular compounds such as ethanolamine and propanediol | acetaldehyde + NH3 | - |
? | |
4.3.1.7 | additional information | Escherichia coli | ethanolamine ammonia-lyase microcompartment is composed of five different shell proteins that have been proposed to assemble into symmetrically shaped polyhedral particles of varying sizes | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.7 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.7 | by nickel-affinity chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.7 | ethanolamine | Escherichia coli has evolved specialized organelles (microcompartments) for the degradation of small molecular compounds such as ethanolamine and propanediol | Escherichia coli | acetaldehyde + NH3 | - |
? | |
4.3.1.7 | additional information | ethanolamine ammonia-lyase microcompartment is composed of five different shell proteins that have been proposed to assemble into symmetrically shaped polyhedral particles of varying sizes | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.1.7 | trimer | determination by gel filtration, freshly prepared protein oligomerizes readily into trimers in the presence or absence of 5 mM beta-mercaptoethanol, monomer consists of 219-amino-acids | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.1.7 | ethanolamine ammonia-lyase | - |
Escherichia coli |
4.3.1.7 | ethanolamine ammonia-lyase BMC | - |
Escherichia coli |
4.3.1.7 | eut-L | - |
Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.7 | 4.6 | 8.5 | crystals have a remarkable high stability with respect to changes in pH | Escherichia coli |