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Literature summary extracted from

  • Elling, R.A.; Fucini, R.V.; Hanan, E.J.; Barr, K.J.; Zhu, J.; Paulvannan, K.; Yang, W.; Romanowski, M.J.
    Structure of the Brachydanio rerio Polo-like kinase 1 (Plk1) catalytic domain in complex with an extended inhibitor targeting the adaptive pocket of the enzyme (2008), Acta Crystallogr. Sect. F, 64, 686-691.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.11.21 medicine Plk1 is a target for the development of anticancer therapeutics Danio rerio

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.21 full-length zPlk1 cDNA and the zPlk1 kinase domain sequence, encompassing residues 17-312, are cloned, the expression plasmid pGEX6P-1 is used Danio rerio

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.11.21 the structure of zPlk1 KD in complex with the inhibitor is solved to 2.85 A resolution Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.21 T196D variant containing an activating substitution Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.21 1-(5-methyl-2-trifluoro-methyl-furan-3-yl)-3-(5-[2-[6-(1H-[1,2,4]triazol-3-ylamino)-pyrimidin-4-ylamino]-ethyl]-thiazol-2-yl)urea
-
 Danio rerio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.21 34390
-
 expected molecular weight of the protein with the N-terminal Gly-Pro-Leu-Gly-Ser linker residues and a T196D substitution Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.21 ATP + protein Danio rerio
-
 ADP + phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.21 Danio rerio Q4KMI8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.21
-
 Danio rerio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.21 ATP + 5FAM-RALMEASFADQAR-NH2 substrate peptide, based on human Cdc25C, used in biochemical activity assay Danio rerio ADP + phosphorylated 5FAM-RALMEASFADQAR-NH2
-
 ?
2.7.11.21 ATP + protein
-
 Danio rerio ADP + phosphoprotein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.21 Plk1
-
 Danio rerio
2.7.11.21 Polo-like kinase 1
-
 Danio rerio
2.7.11.21 zPlk1
-
 Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.21 7.2
-
 activity assay Danio rerio