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Literature summary extracted from

  • Pasquo, A.; Bonamore, A.; Franceschini, S.; Macone, A.; Boffi, A.; Ilari, A.
    Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum (2008), Acta Crystallogr. Sect. F, 64, 281-283.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.78 the NCS protein truncated at the first 19 amino acids with an N-terminal MTGS sequence and a His tag at the C-terminus and its SeMet-substituted variant are subcloned into the NdeI and XhoI restriction sites of the vector pET22b. Transformation of chemically competent Escherichia coli strain BL21DE is performed and transformed cells are plated onto LB-ampicillin plates Thalictrum flavum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.78 at 294 K by the hanging-drop vapour-diffusion method using ammonium sulfate and sodium chloride as precipitant agents and diffract to better than 3.0 A resolution using a synchrotron-radiation source. The crystals belong to the trigonal space group P3121, with unit-cell parameters a = b = 86.31, c = 118.36 A. A selenomethionine derivate is crystallized in the same space group, 2.7 A resolution Thalictrum flavum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.78 4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde Thalictrum flavum
-
 (S)-Norcoclaurine + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.78 Thalictrum flavum Q67A25
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.78 HisTrap Fast Flow column Thalictrum flavum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.78 4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde
-
 Thalictrum flavum (S)-Norcoclaurine + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.78 NCS
-
 Thalictrum flavum
4.2.1.78 norcoclaurine synthase
-
 Thalictrum flavum