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Literature summary extracted from

  • Misra, G.; Aggarwal, A.; Mittal, S.; Singh, Y.; Ramachandran, R.
    Purification, crystallization and preliminary structural analysis of nucleoside diphosphate kinase from Bacillus anthracis (2007), Acta Crystallogr. Sect. F, 63, 1084-1086.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.6 hanging drop vapour-diffusion method, 0.002 ml protein solution consisting of 10 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM EDTA equilibrated against 0.5 ml reservoir solution consisting of 2.25 M ammonium formate and 0.1 M HEPES buffer, pH 7.25, X-ray diffraction structure determination at 2.0 A resolution Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+
-
 Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 additional information Bacillus anthracis the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Bacillus anthracis Q81SV8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 additional information the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor Bacillus anthracis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDK
-
 Bacillus anthracis
2.7.4.6 nucleoside diphosphate kinase
-
 Bacillus anthracis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.6 22
-
 assay at room temperature Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.6 7.4
-
 assay at Bacillus anthracis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.6 ATP
-
 Bacillus anthracis