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Literature summary extracted from

  • Parsons, J.F.; Shi, K.M.; Ladner, J.E.
    Structure of isochorismate synthase in complex with magnesium (2008), Acta Crystallogr. Sect. D, 64, 607-610.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.4.2 expressed in Escherichia coli strain BL21 (DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.4.2 enzyme without Mg2+, hanging drop vapour diffusion method, using 0.2 M trisodium citrate, 6% glycerol, 8% PEG 3350, enzyme in complex with Mg2+, sitting drop vapour diffusion method, using 0.1 M bis-tris pH 6.5, 20% PEG MME 5000 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.4.2 Mg2+ required for catalysis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.4.4.2 Escherichia coli P38051
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.4.2 Co2+-ion affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.4.2 Chorismate
-
 Escherichia coli Isochorismate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
5.4.4.2 menaquinone-specific isochorismate synthase
-
 Escherichia coli
5.4.4.2 MenF
-
 Escherichia coli