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Literature summary extracted from

  • Letts, J.A.; Persson, M.; Schuman, B.; Borisova, S.N.; Palcic, M.M.; Evans, S.V.
    The effect of heavy atoms on the conformation of the active-site polypeptide loop in human ABO(H) blood-group glycosyltransferase B (2007), Acta Crystallogr. Sect. D, 63, 860-865.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.37 mutant GTB C209A is crystallized in both the presence and the absence of mercury, 0.01 ml drops containing 6-8 mg/ml GTB, 70 mM N-(2-acetamido)-2-iminodiacetic acid, pH 7.5, 50 mM sodium acetate, pH 4.6, 40 mM NaCl, 5-8 mM MnCl2, 2.5% v/v 2-methyl-2,4-pentanediol, 5% v/v glycerol, 2% w/v PEG 4000 and 0.3-0.5 mM 3-chloromercuri-2-methoxypropylurea is suspended over 1 ml reservoir solution containing 50 mM N-(2-acetamido)-2-iminodiacetic acid, pH 7.5, 10 mM, MnCl2, 100 mM ammonium sulfate, 5% v/v MPD, 10% v/v glycerol and 8-10% w/v PEG 4000. Growing crystals of native GTB in the absence of mercury using protein concentrations of 6-8 mg /ml are unsuccessful, therefore crystals of the C209A mutant are grown from protein concentrations of over 30 mg/ml with the lowest observed concentration that yielded diffraction-quality crystals being 15 mg/ml 5-8 ml drops containing 1% PEG 4000, 4.5% MPD, 0.1 M ammonium sulfate, 0.07 M NaCl, 0.05 M N-(2-acetamido)-2-iminodiacetic acid, pH 7.5, and 5 mM CoCl2 are stored at 4-6°C for 3-5 days over a reservoir of 2.7% PEG 4000, 7% MPD, 0.32 M ammonium sulfate, 0.25 M NaCl and 0.2 M N-(2-acetamido)-2-iminodiacetic acid. Both sets of crystals are washed with mother liquor containing 6-7% PEG 4000, 70 mM N-(2-acetamido)-2-iminodiacetic acid, pH 7.5, 30 mM sodium acetate, pH 4.6, 40 mM ammonium sulfate, 29-30% glycerol and 9-10 mM MnCl2 or 5 mM CoCl2 for the heavy-metal derivative or native protein, respectively. X-ray diffraction structure determination and analysis at 1.8-2.4 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.37 C209A site-directed mutagenesis, mutant structure determination Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.37 cell surface
-
Homo sapiens 9986
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.37 Hg structures of the mercury derivative of GTB and GTB C209A, mercury does not affect the conformation of active-site residues, the structure of GTB C209A demonstrates that the mercury coordinated by Cys209 is partially responsible for the disorder of the 16-amino-acid internal loop observed in the heavy-atom structure of GTB Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens P16442
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.37 erythrocyte
-
Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 ABO(H) blood-group glycosyltransferase B
-
Homo sapiens
2.4.1.37 alpha(1-3)galactosyltransferase
-
Homo sapiens
2.4.1.37 GTB
-
Homo sapiens