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Literature summary extracted from

  • Dolashki, A.; Abrashev, R.; Stevanovic, S.; Stefanova, L.; Ali, S.A.; Velkova, L.; Hristova, R.; Angelova, M.; Voelter, W.; Devreese, B.; Van Beeumen, J.; Dolashka-Angelova, P.
    Biochemical properties of Cu/Zn-superoxide dismutase from fungal strain Aspergillus niger 26 (2008), Spectrochim. Acta A. Mol. Biomol. Spectrosc., 71, 975-983.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a Cu,Zn-SOD Aspergillus niger
1.15.1.1 Mn2+ a Mn-SOD Aspergillus niger
1.15.1.1 Zn2+ a Cu,Zn-SOD Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 15821
-
 2 * 15821, Cu,Zn-SOD, sequence calculation Aspergillus niger
1.15.1.1 22340
-
 4 * 22340, Mn-SOD, sequence calculation Aspergillus niger
1.15.1.1 30000
-
 Cu,Zn-SOD, native PAGE Aspergillus niger
1.15.1.1 88000
-
 Mn-SOD, native PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 O2.- + H+ Aspergillus niger
-
 O2 + H2O2
-
 ?
1.15.1.1 O2.- + H+ Aspergillus niger 26
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Aspergillus niger
-
-
-
1.15.1.1 Aspergillus niger 26
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native Cu,Zn-SOD about 13fold by two steps of hydrophobic interaction chromatography and two steps of anion exchange chromatography Aspergillus niger

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.1 2 superoxide + 2 H+ = O2 + H2O2 the amino acid residues His46, His48, His63, His71, His80, and His120, and Asp83 in the active site are conserved as in other Cu/ZnSODs Aspergillus niger
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 2940
-
 purified Cu,Zn-SOD Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 additional information SOD activity measurement using the nitroblue tetrazolium Aspergillus niger ?
-
 ?
1.15.1.1 additional information SOD activity measurement using the nitroblue tetrazolium Aspergillus niger 26 ?
-
 ?
1.15.1.1 O2.- + H+
-
 Aspergillus niger O2 + H2O2
-
 ?
1.15.1.1 O2.- + H+
-
 Aspergillus niger 26 O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 15821, Cu,Zn-SOD, sequence calculation Aspergillus niger
1.15.1.1 More peptide mapping by tryptic digest and amino acid sequence determination and analysis, the enzyme does neither contain a Tyr residue nor a carbohydrate chain occupying an N-linkage site -N-I-Y-,overview, homology modeling of Cu/Zn-SOD Aspergillus niger
1.15.1.1 tetramer 4 * 22340, Mn-SOD, sequence calculation Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Cu/Zn-SOD
-
 Aspergillus niger
1.15.1.1 Cu/Zn-superoxide dismutase
-
 Aspergillus niger
1.15.1.1 Mn-SOD
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.15.1.1 25 85 at pH 7.8, temperature profile, overview Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 60
-
 30 min, 53% remaining activity Aspergillus niger
1.15.1.1 85
-
 30 min, 15% remaining activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 6.2
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 4 12 90% of maximal activity at pH 5.6-pH 7.5, 15% at pH 4.0, 60% at pH 12.0, 25°C, pH profile, overview Aspergillus niger