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Literature summary extracted from

  • Beknazarov, B.O.; Valikhanov, M.N.
    Some characteristics of cotton pyrophosphatase activation by magnesium (2006), Russ. J. Plant Physiol., 53, 48-53.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Ca2+ can displace Mg2+ Gossypium hirsutum
3.6.1.1 Mg2+ activates by decreasing the value of the Michaelis-Menten constant, dependent on, highest activity at pH 7.4 and 5.0 mM, Mg2+ shifts the pH optimum, Mg2+ associates rather weakly with the pyrophosphatase active center and can be readily displaced by Ca2+ , overview Gossypium hirsutum

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Gossypium hirsutum
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 cv. 108-F
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 native enzyme from germinated seeds by acetone precipitation, gel ifltration, and anion exchange chromatography, 51.7fold Gossypium hirsutum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.1 seed germinating, presence of Mg2+ ions in the germination medium markedly shortens time for the attainment of the diphosphatase maximum activity, overview Gossypium hirsutum
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 415
-
 purified native enzyme Gossypium hirsutum

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 pyrophosphatase
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 Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 7.2 8.6 broad optimum, the pH optimum shifts with binding of Mg2+, overview Gossypium hirsutum