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Literature summary extracted from

  • Anikina, O.M.; Semashko, T.A.; Oksenoit, E.S.; Lysogorskaya, E.N.; Filippova, I.Y.
    Native subtilisin Karlsberg and modified subtilisin 72 as effective catalysts of peptide bond formation in organic media (2006), Russ. J. Bioorg. Chem., 32, 116-121.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.21.62 additional information the subtilisin Karlsberg complex with sodium dodecyl sulfate in anhydrous organic solvents is an effective catalyst of peptide synthesis with multifunctional amino acids in positions P 1 and (Glu, Arg, and Asp) containing unprotected side ionogenic groups Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.13
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMSO Bacillus subtilis
3.4.21.62 0.14
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus subtilis
3.4.21.62 0.18
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus subtilis
3.4.21.62 0.19
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus subtilis
3.4.21.62 0.25
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus licheniformis
3.4.21.62 0.25
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus licheniformis
3.4.21.62 4
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.62 Ca2+
-
 Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus licheniformis
-
-
-
3.4.21.62 Bacillus subtilis
-
-
-
3.4.21.62 Bacillus subtilis 72
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 Glp-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 Glp-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus licheniformis ?
-
 ?
3.4.21.62 Glp-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis 72 ?
-
 ?
3.4.21.62 Z-Ala-Ala-Leu-OCH3 + H2O
-
 Bacillus licheniformis ?
-
 ?
3.4.21.62 Z-Ala-Ala-Leu-OMe + Phe-p-nitroanilide
-
 Bacillus licheniformis Z-Ala-Ala-Leu-Phe-p-nitroanilide + methanol
-
 ?
3.4.21.62 Z-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 Z-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus licheniformis ?
-
 ?
3.4.21.62 Z-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis 72 ?
-
 ?
3.4.21.62 Z-Thr-Ala-Thr-OCH3 + Asp-p-nitroanilide
-
 Bacillus licheniformis Z-Thr-Ala-Thr-Asp-p-nitroanilide + methanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 subtilisin 72
-
 Bacillus subtilis
3.4.21.62 subtilisin Karlsberg
-
 Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 64.3
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus subtilis
3.4.21.62 69
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus licheniformis
3.4.21.62 70.9
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus subtilis
3.4.21.62 77
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus licheniformis
3.4.21.62 80
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMSO Bacillus subtilis
3.4.21.62 100
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus subtilis
3.4.21.62 104
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus licheniformis