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Literature summary extracted from

  • Yeo, C.C.; Tan, C.L.; Gao, X.; Zhao, B.; Poh, C.L.
    Characterization of hbzE-encoded gentisate 1,2-dioxygenase from Pseudomonas alcaligenes NCIMB 9867 (2007), Res. Microbiol., 158, 608-616.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 overexpressed as a hexahistidine fusion protein from Escherichia coli Pseudomonas alcaligenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.4 Ca2+ 5 mM, partial inhibition Pseudomonas alcaligenes
1.13.11.4 Cu2+ 5 mM, complete inhibition Pseudomonas alcaligenes
1.13.11.4 EDTA 5 mM, more than 95% inhibition Pseudomonas alcaligenes
1.13.11.4 Fe2+ 1 mM, complete inactivation Pseudomonas alcaligenes
1.13.11.4 Hg2+ 5 mM, more than 95% inhibition Pseudomonas alcaligenes
1.13.11.4 Mn2+ 5 mM, partial inhibition Pseudomonas alcaligenes
1.13.11.4 Na+ 5 mM, partial inhibition Pseudomonas alcaligenes
1.13.11.4 Zn2+ 5 mM, more than 95% inhibition Pseudomonas alcaligenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.01155
-
 3-Ethylgentisate pH 7.4, 23°C Pseudomonas alcaligenes
1.13.11.4 0.029
-
 3-Bromogentisate pH 7.4, 23°C Pseudomonas alcaligenes
1.13.11.4 0.0313
-
 3-Isopropylgentisate pH 7.4, 23°C Pseudomonas alcaligenes
1.13.11.4 0.0505
-
 3-Fluorogentisate pH 7.4, 23°C Pseudomonas alcaligenes
1.13.11.4 0.0549
-
 3-Methylgentisate pH 7.4, 23°C Pseudomonas alcaligenes
1.13.11.4 0.113
-
 2,5-Dihydroxybenzoate pH 7.4, 23°C Pseudomonas alcaligenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.4 Fe2+ 0.05-0.1 mM, 160% increase in activity Pseudomonas alcaligenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.4 40000
-
 x * 40000, SDS-PAGE Pseudomonas alcaligenes
1.13.11.4 150000
-
 non-denaturing PAGE Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.4 additional information Pseudomonas alcaligenes GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate ?
-
 ?
1.13.11.4 additional information Pseudomonas alcaligenes P25X GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Pseudomonas alcaligenes
-
-
-
1.13.11.4 Pseudomonas alcaligenes P25X
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4 recombinant enzyme Pseudomonas alcaligenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2 i.e. gentisate Pseudomonas alcaligenes maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2 i.e. gentisate Pseudomonas alcaligenes P25X maleylpyruvate
-
 ?
1.13.11.4 3-bromogentisate + O2 Vmax is 48.5% of Vmax for gentisate Pseudomonas alcaligenes 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
1.13.11.4 3-ethylgentisate + O2 Vmax is 39.7% of Vmax for gentisate Pseudomonas alcaligenes 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
1.13.11.4 3-ethylgentisate + O2 Vmax is 39.7% of Vmax for gentisate Pseudomonas alcaligenes P25X 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
1.13.11.4 3-fluorogentisate + O2 Vmax of recombinant enzyme is 20.5% higher compared to gentisate Pseudomonas alcaligenes 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
1.13.11.4 3-fluorogentisate + O2 Vmax of recombinant enzyme is 20.5% higher compared to gentisate Pseudomonas alcaligenes P25X 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
1.13.11.4 3-isopropylgentisate + O2 Vmax is 57.1% of Vmax for gentisate Pseudomonas alcaligenes 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
 ?
1.13.11.4 3-methylgentisate + O2 Vmax is 10.1% of Vmax for gentisate Pseudomonas alcaligenes 3-methyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
1.13.11.4 additional information GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate Pseudomonas alcaligenes ?
-
 ?
1.13.11.4 additional information GDO-II is unable to utilize gentisate that is substituted at the carbon-4 position (i.e., 4-chloro- and 4-methylgentisate) Pseudomonas alcaligenes ?
-
 ?
1.13.11.4 additional information GDO-II is strictly inducible by aromatic substrates such as 3-hydroxybenzoate and gentisate Pseudomonas alcaligenes P25X ?
-
 ?
1.13.11.4 additional information GDO-II is unable to utilize gentisate that is substituted at the carbon-4 position (i.e., 4-chloro- and 4-methylgentisate) Pseudomonas alcaligenes P25X ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.4 ? x * 40000, SDS-PAGE Pseudomonas alcaligenes

Synonyms

EC Number Synonyms Comment Organism
1.13.11.4 GDO-II
-
 Pseudomonas alcaligenes
1.13.11.4 hbzE
-
 Pseudomonas alcaligenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.4 25
-
-
 Pseudomonas alcaligenes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.4 15
-
 no activity below Pseudomonas alcaligenes
1.13.11.4 60
-
 no activity above Pseudomonas alcaligenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.4 7 8
-
 Pseudomonas alcaligenes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.4 Pseudomonas alcaligenes pH range 3.5-9.5 4.7 4.5