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Literature summary extracted from

  • Carbone, V.; Endo, S.; Sumii, R.; Chung, R.P.; Matsunaga, T.; Hara, A.; El-Kabbani, O.
    Structures of dimeric dihydrodiol dehydrogenase apoenzyme and inhibitor complex: probing the subunit interface with site-directed mutagenesis (2007), Proteins Struct. Funct. Bioinform., 70, 176-187.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.20 expressed in Escherichia coli BL21(DE3) cells Macaca fascicularis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.20 vapour diffusion method with 2 M ammonium phosphate and 0.1 M sodium citrate buffer (pH 5.6) and 20% glycerol Macaca fascicularis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.20 R148A activity and temperature stability are similar to the wild type enzyme Macaca fascicularis
1.3.1.20 R148A/R202A very low activity, the Vmax value of the mutant enzyme is 3.7fold lower than the wild type enzyme, displays significant temperature sensitivity Macaca fascicularis
1.3.1.20 R202A activity and temperature stability are similar to the wild type enzyme Macaca fascicularis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.20 4-hydroxyacetophenone
-
 Macaca fascicularis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.20 Macaca fascicularis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.20 ammonium sulfate precipitation, Red Sepharose column chromatography, and Sephadex G-100 gel filtration Macaca fascicularis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.20 D-xylose + NADP+
-
 Macaca fascicularis D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
 Macaca fascicularis catechol + NAD(P)H
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.20 dimer X-ray crystallography, the intact dimer is not essential for catalytic activity Macaca fascicularis

Synonyms

EC Number Synonyms Comment Organism
1.3.1.20 dihydrodiol dehydrogenase
-
 Macaca fascicularis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.20 37
-
 the wild type enzyme and mutant enzymes R148A and R202A are stable at 37°C Macaca fascicularis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.20 NADP+ dependent on Macaca fascicularis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.20 0.00097
-
 wild type enzyme Macaca fascicularis 4-hydroxyacetophenone