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Literature summary extracted from

  • Angelucci, F.; Miele, A.E.; Boumis, G.; Dimastrogiovanni, D.; Brunori, M.; Bellelli, A.
    Glutathione reductase and thioredoxin reductase at the crossroad: the structure of Schistosoma mansoni thioredoxin glutathione reductase (2008), Proteins, 72, 936-945.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 expressed in Escherichia coli BL21(DE3) cells Schistosoma mansoni
1.8.1.9 expressed in Escherichia coli BL21(DE3) cells Schistosoma mansoni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.B1 crystal structure at 2.2 A resolution of TGR, deleted in the last two residues Schistosoma mansoni
1.8.1.7 hanging drop vapor diffusion method, using 22% (w/v) PEG 3350, 0.1 M HEPES pH 7.2, 0.2 M NaNO3, 5 mM dithiothreitol, or beta-mercaptoethanol Schistosoma mansoni
1.8.1.9 hanging drop vapor diffusion method, using 22% (w/v) PEG 3350, 0.1 M HEPES pH 7.2, 0.2 M NaNO3, 5 mM dithiothreitol, or beta-mercaptoethanol Schistosoma mansoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 0.205
-
 GSSG
-
 Schistosoma mansoni
1.8.1.B1 0.71
-
 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Schistosoma mansoni
1.8.1.7 0.0715
-
 GSSG truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 150 mM potassium phosphate pH 7.0, at 37°C Schistosoma mansoni
1.8.1.7 0.114
-
 5,5'-dithiobis(2-nitrobenzoic acid) full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.7 0.205
-
 GSSG full length enzyme, in 150 mM potassium phosphate pH 7.0, at 37°C Schistosoma mansoni
1.8.1.7 0.71
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.9 0.00637
-
 thioredoxin disulfide full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.9 0.114
-
 5,5'-dithiobis(2-nitrobenzoic acid) full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.9 0.71
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 90000
-
 SDS-PAGE Schistosoma mansoni
1.8.1.9 90000
-
 SDS-PAGE Schistosoma mansoni

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Schistosoma mansoni
-
-
-
1.8.1.7 Schistosoma mansoni
-
-
-
1.8.1.9 Schistosoma mansoni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Schistosoma mansoni
1.8.1.9
-
 Schistosoma mansoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Schistosoma mansoni 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.B1 GSSG + NADPH + H+
-
 Schistosoma mansoni glutathione + NADP+
-
 ?
1.8.1.7 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Schistosoma mansoni 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.7 GSSG + NADPH + H+
-
 Schistosoma mansoni GSH + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Schistosoma mansoni 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Schistosoma mansoni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 90000, SDS-PAGE Schistosoma mansoni
1.8.1.9 dimer 2 * 90000, SDS-PAGE Schistosoma mansoni

Synonyms

EC Number Synonyms Comment Organism
1.8.1.B1 SmTGR
-
 Schistosoma mansoni
1.8.1.B1 thioredoxin glutathione reductase
-
 Schistosoma mansoni
1.8.1.7 TGR
-
 Schistosoma mansoni
1.8.1.7 thioredoxin glutathione reductase chimeric enzyme able to transport reducing equivalents from NADPH to both GSH/GSSG and thioredoxin systems via the fusion of the thioredxin reductase and glutathione reductase domains Schistosoma mansoni
1.8.1.9 TGR
-
 Schistosoma mansoni
1.8.1.9 thioredoxin glutathione reductase chimeric enzyme able to transport reducing equivalents from NADPH to both GSH/GSSG and thioredoxin systems via the fusion of the thioredxin reductase and glutathione reductase domains Schistosoma mansoni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.B1 0.19
-
 GSSG
-
 Schistosoma mansoni
1.8.1.B1 1.2
-
 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Schistosoma mansoni
1.8.1.7 0.19
-
 GSSG truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 150 mM potassium phosphate pH 7.0, at 37°C Schistosoma mansoni
1.8.1.7 1.2
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.7 16
-
 5,5'-dithiobis(2-nitrobenzoic acid) full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.9 1.2
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.9 16
-
 5,5'-dithiobis(2-nitrobenzoic acid) full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
1.8.1.9 30
-
 thioredoxin disulfide full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD
-
 Schistosoma mansoni
1.8.1.7 NADPH
-
 Schistosoma mansoni
1.8.1.9 FAD
-
 Schistosoma mansoni
1.8.1.9 NADPH
-
 Schistosoma mansoni