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Literature summary extracted from

  • Freydank, A.C.; Brandt, W.; Draeger, B.
    Protein structure modeling indicates hexahistidine-tag interference with enzyme activity (2008), Proteins, 72, 173-183.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.206 gene trI, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) Solanum dulcamara
1.1.1.236 gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview Solanum dulcamara

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.236 additional information
-
 additional information the recombinant His-tagged enzyme shows an increased substrate affinity compared to the wild-type enzyme Solanum dulcamara

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.236 29000
-
 x * 29000, recombinant His-tagged enzyme, SDS-PAGE Solanum dulcamara

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.206 tropinone + NADPH + H+ Solanum dulcamara the enzyme is involved in tropane alkaloid biosynthesis, e.g. of (S)-hyoscyamine, overview tropine + NADP+
-
 r
1.1.1.236 tropinone + NADPH + H+ Solanum dulcamara the enzyme is involved in tropane alkaloid biosynthesis, overview pseudotropine + NADP+ leading to formation of calystegines ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.206 Solanum dulcamara
-
 gene trI
-
1.1.1.236 Solanum dulcamara
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.206 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Solanum dulcamara
1.1.1.236 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Solanum dulcamara

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.206 tropinone + NADPH + H+
-
 Solanum dulcamara tropine + NADP+
-
 r
1.1.1.206 tropinone + NADPH + H+ the enzyme is involved in tropane alkaloid biosynthesis, e.g. of (S)-hyoscyamine, overview Solanum dulcamara tropine + NADP+
-
 r
1.1.1.236 additional information no activity with quinuclidione and 8-thiabicyclo[3.2.1]octan-2-one Solanum dulcamara ?
-
 ?
1.1.1.236 tropinone + NADPH + H+ the enzyme is involved in tropane alkaloid biosynthesis, overview Solanum dulcamara pseudotropine + NADP+ leading to formation of calystegines ?
1.1.1.236 tropinone + NADPH + H+ active site structure and catalytic triad, the recombinant His-tagged enzyme shows 4fold higher activity compared to the wild-type enzyme, overview Solanum dulcamara pseudotropine + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.236 ? x * 29000, recombinant His-tagged enzyme, SDS-PAGE Solanum dulcamara
1.1.1.236 More structure modelling, overview Solanum dulcamara

Synonyms

EC Number Synonyms Comment Organism
1.1.1.206 More the enzyme belongs to the family of short chain dehydrogenases Solanum dulcamara
1.1.1.206 TRI
-
 Solanum dulcamara
1.1.1.236 More the enzyme belongs to the family of short chain dehydrogenases Solanum dulcamara
1.1.1.236 pseudotropinone forming tropinone reductase
-
 Solanum dulcamara
1.1.1.236 TRII
-
 Solanum dulcamara

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.236 30
-
 assay at Solanum dulcamara

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.236 6.4
-
 recombinant His-tagged enzyme Solanum dulcamara

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.236 additional information
-
 the recombinant His-tagged enzyme shows a broader pH spectrum compared to the wild-type enzyme Solanum dulcamara

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.206 NADP+
-
 Solanum dulcamara
1.1.1.206 NADPH
-
 Solanum dulcamara
1.1.1.236 NADPH binding site sequence, overview Solanum dulcamara