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Literature summary extracted from

  • Kim, S.H.; Hisano, T.; Iwasaki, W.; Ebihara, A.; Miki, K.
    Crystal structure of the flavin reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8: Structural basis for the flavin affinity (2008), Proteins, 70, 718-730.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.9 flavin reductase component HpaC of 4-hydroxyphenylacetate 3-monooxygenase in a ternary complex with FAD and NAD+, mixing of 0.002-0.005 ml of a solution containing 25 mg/mL HpaCTt-FAD, 5 mM NADH, and 1 mM dithiothreitol with an equal volume of a reservoir solution containing 20% w/v PEG 1000, 10% w/v PEG 8000, and 10% v/v glycerol, and then equilibrating the solution against the reservoir solution, 2 days, X-ray diffraction structure determination and analysis at 1.65-3.3 A resolution Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.9 4-hydroxyphenylacetate + NAD(P)H + H+ + O2 Thermus thermophilus
-
 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NAD(P)H + H+ + O2 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.9 Thermus thermophilus
-
-
-
1.14.14.9 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.9 4-hydroxyphenylacetate + NAD(P)H + H+ + O2
-
 Thermus thermophilus 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NAD(P)H + H+ + O2 FAD and NAD+ are bound in the groove in the extended and folded conformation, respectively, structure, overview Thermus thermophilus 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NAD(P)H + H+ + O2
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NAD(P)H + H+ + O2 FAD and NAD+ are bound in the groove in the extended and folded conformation, respectively, structure, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.9 30
-
 assay at Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.9 7
-
 assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.9 FAD FAD is not tightly bound to HpaC, the flavin reductase component of the enzyme, and is bound in the groove in the extended and folded conformation, binding site structure, overview Thermus thermophilus
1.14.14.9 FMN can substitute for FAD in the enzyme assay Thermus thermophilus
1.14.14.9 NAD(P)H product NAD+ is bound in the groove in the extended and folded conformation, binding site structure, overview Thermus thermophilus