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Literature summary extracted from
- Identification of fungal oxaloacetate hydrolyase within the isocitrate lyase/PEP mutase enzyme superfamily using a sequence marker-based method (2008), Proteins, 70, 157-166.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.7.1.1 | into the vector pET-3c | Dianthus caryophyllus |
| 3.7.1.1 | into the vector pET-3c | Botrytis cinerea |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.1 | 0.022 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260P | Botrytis cinerea |  |
| 3.7.1.1 | 0.054 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260A | Botrytis cinerea | |
| 3.7.1.1 | 0.065 | - |
oxaloacetate | wild-type protein | Botrytis cinerea | |
| 3.7.1.1 | 0.13 | - |
oxaloacetate | wild-type protein | Dianthus caryophyllus | |
| 3.7.1.1 | 0.14 | - |
(2R,3S)-2,3-dimethylmalate | wild-type protein | Botrytis cinerea | |
| 3.7.1.1 | 0.15 | - |
oxaloacetate | mutant S260P, trial 1 | Botrytis cinerea | |
| 3.7.1.1 | 0.22 | - |
oxaloacetate | mutant S260P, trial 2 | Botrytis cinerea | |
| 3.7.1.1 | 0.53 | - |
(2R,3S)-2-ethyl-3-methylmalate | wild-type protein | Dianthus caryophyllus | |
| 3.7.1.1 | 0.56 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257A | Dianthus caryophyllus | |
| 3.7.1.1 | 0.66 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257P | Dianthus caryophyllus | |
| 3.7.1.1 | 0.7 | - |
oxaloacetate | mutant S260A, trial 1 | Botrytis cinerea | |
| 3.7.1.1 | 0.84 | - |
oxaloacetate | mutant S257P | Dianthus caryophyllus | |
| 3.7.1.1 | 1.307 | - |
oxaloacetate | mutant S260A, trial 2 | Botrytis cinerea | |
| 3.7.1.1 | 1.53 | - |
oxaloacetate | mutant S257A | Dianthus caryophyllus | |
| 3.7.1.1 | 2.6 | - |
oxaloacetate | mutant S257T, trial 2 | Dianthus caryophyllus | |
| 3.7.1.1 | 3.6 | - |
oxaloacetate | mutant S257T, trial 1 | Dianthus caryophyllus | |
| 3.7.1.1 | 7 | - |
oxaloacetate | mutant S260T, trial 1 | Botrytis cinerea | |
| 3.7.1.1 | 7.7 | - |
oxaloacetate | mutant S260T, trial 2 | Botrytis cinerea | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.1 | (2R,3S)-2,3-dimethylmalate | Botrytis cinerea | - |
pyruvate + propionic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.1 | Botrytis cinerea | Q6PNM8 | - |
- |
| 3.7.1.1 | Dianthus caryophyllus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.7.1.1 | proteins are purified to homogeneity | Dianthus caryophyllus |
| 3.7.1.1 | proteins are purified to homogeneity | Botrytis cinerea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.1 | (2R,3S)-2,3-dimethylmalate | - |
Botrytis cinerea | pyruvate + propionic acid | - |
? | |
| 3.7.1.1 | (2R,3S)-2-ethyl-3-methylmalate + H2O | - |
Dianthus caryophyllus | ? | - |
? | |
| 3.7.1.1 | oxaloacetate + H2O | - |
Dianthus caryophyllus | oxalate + acetate | - |
? | |
| 3.7.1.1 | oxaloacetate + H2O | - |
Botrytis cinerea | oxalate + acetate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.7.1.1 | OAH | - |
Botrytis cinerea |
| 3.7.1.1 | OAH-active petal death protein | - |
Dianthus caryophyllus |
| 3.7.1.1 | oxaloacetase | - |
Botrytis cinerea |
| 3.7.1.1 | oxaloacetate acetylhydrolase | - |
Botrytis cinerea |
| 3.7.1.1 | oxaloacetate hydrolase | - |
Botrytis cinerea |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.1 | 25 | - |
activity assay | Dianthus caryophyllus |
| 3.7.1.1 | 25 | - |
activity assay | Botrytis cinerea |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.1 | 0.0249 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260A | Botrytis cinerea | |
| 3.7.1.1 | 0.0293 | - |
(2R,3S)-2,3-dimethylmalate | wild-type protein | Botrytis cinerea | |
| 3.7.1.1 | 0.074 | - |
(2R,3S)-2,3-dimethylmalate | mutant S260P | Botrytis cinerea | |
| 3.7.1.1 | 0.71 | - |
oxaloacetate | mutant S260A, trial 2 | Botrytis cinerea | |
| 3.7.1.1 | 0.83 | - |
oxaloacetate | mutant S260A, trial 1 | Botrytis cinerea | |
| 3.7.1.1 | 0.96 | - |
oxaloacetate | mutant S260P, trial 1 | Botrytis cinerea | |
| 3.7.1.1 | 0.98 | - |
oxaloacetate | mutant S260P, trial 2 | Botrytis cinerea | |
| 3.7.1.1 | 1.14 | - |
oxaloacetate | mutant S257T, trial 1 | Dianthus caryophyllus | |
| 3.7.1.1 | 1.16 | - |
oxaloacetate | mutant S257T, trial 2 | Dianthus caryophyllus | |
| 3.7.1.1 | 2.59 | - |
oxaloacetate | mutant S260T, trial 1 | Botrytis cinerea | |
| 3.7.1.1 | 2.72 | - |
oxaloacetate | wild-type protein | Dianthus caryophyllus | |
| 3.7.1.1 | 2.91 | - |
oxaloacetate | mutant S257A | Dianthus caryophyllus | |
| 3.7.1.1 | 3.6 | - |
oxaloacetate | mutant S260T, trial 2 | Botrytis cinerea | |
| 3.7.1.1 | 4.1 | - |
oxaloacetate | mutant S257P | Dianthus caryophyllus | |
| 3.7.1.1 | 5.4 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257A | Dianthus caryophyllus | |
| 3.7.1.1 | 8.3 | - |
(2R,3S)-2-ethyl-3-methylmalate | mutant S257P | Dianthus caryophyllus | |
| 3.7.1.1 | 8.4 | - |
(2R,3S)-2-ethyl-3-methylmalate | wild-type protein | Dianthus caryophyllus | |
| 3.7.1.1 | 17.4 | - |
oxaloacetate | wild-type protein | Botrytis cinerea | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.1 | 7.5 | - |
activity assay | Dianthus caryophyllus |
| 3.7.1.1 | 7.5 | - |
activity assay | Botrytis cinerea |
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