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Literature summary extracted from

  • Iwanami, K.; Uda, K.; Tada, H.; Suzuki, T.
    Cytoplasmic and mitochondrial creatine kinases from the skeletal muscle of sperm whale (Physeter macrocephalus). Molecular cloning and enzyme characterization (2008), Protein J., 27, 43-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2 expressed in Escherichia coli as a fusion protein with maltose-binding protein, mitochondrial isoform sMiCK Physeter catodon
2.7.3.2 expressed in Escherichia coli as a fusion protein with maltose-binding protein, muscle-type isoform MCK Physeter catodon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 1.01
-
 ATP
-
 Physeter catodon
2.7.3.2 4.3
-
 Creatine mitochondrial isoform sMiCK Physeter catodon
2.7.3.2 9.38
-
 Creatine
-
 Physeter catodon
2.7.3.2 9.38
-
 ATP mitochondrial isoform sMiCK Physeter catodon

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.2 cytosol cytoplasmic, muscle-type isoform MCK Physeter catodon 5829
-
2.7.3.2 mitochondrion sarcomeric, mitochondrial isoform sMiCK Physeter catodon 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Physeter catodon
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 skeletal muscle
-
 Physeter catodon
-
2.7.3.2 skeletal muscle sMiCK Physeter catodon
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ADP + phosphocreatine synergistic substrate binding, mitochondrial isoform sMiCK Physeter catodon ATP + creatine
-
 ?
2.7.3.2 ADP + phosphocreatine synergistic substrate binding, muscle-type isoform MCK Physeter catodon ATP + creatine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 MCK
-
 Physeter catodon
2.7.3.2 sMiCK
-
 Physeter catodon

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.2 36.4
-
 ATP muscle-type isoform MCK Physeter catodon
2.7.3.2 36.4
-
 Creatine muscle-type isoform MCK Physeter catodon
2.7.3.2 38.4
-
 ATP mitochondrial isoform sMiCK Physeter catodon
2.7.3.2 38.4
-
 Creatine mitochondrial isoform sMiCK Physeter catodon