Literature summary extracted from
Goren, M.A.; Fox, B.G.
Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex (2008), Protein Expr. Purif., 62, 171-178.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.19.1 |
in vitro translation using a wheat germ cell-free extract in the presence of unilamelar liposomes, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.19.1 |
additional information |
near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, overview |
Homo sapiens |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.19.1 |
Fe2+ |
heme and non-heme iron |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.19.1 |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ |
Homo sapiens |
- |
oleoyl-CoA + ferricytochrome b5 + H2O |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.19.1 |
Homo sapiens |
O00767 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.19.1 |
near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes, overview |
Homo sapiens |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
1.14.19.1 |
in vitro reconstitution of the non-heme iron and heme active sites to a functional reconstituted enzyme complex after in vitro translation and protein integration into liposomes, method optimization, overview |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.19.1 |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ |
- |
Homo sapiens |
oleoyl-CoA + ferricytochrome b5 + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.19.1 |
SCD |
- |
Homo sapiens |
1.14.19.1 |
stearoyl-CoA desaturase |
- |
Homo sapiens |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.19.1 |
37 |
- |
assay at |
Homo sapiens |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.19.1 |
7.2 |
- |
assay at |
Homo sapiens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.19.1 |
ferrocytochrome b5 |
- |
Homo sapiens |
|