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Literature summary extracted from

  • Lee, S.K.; Keasling, J.D.
    Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methods (2008), Protein Expr. Purif., 61, 197-203.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.140 expressed in Escherichia coli from the Salmonella enterica prpBCDE promoter, comparison to expression products derived from the strongest IPTG-inducible promoter PT7, DH10B strain used for DNA-manipulations, BLRDE3 strain host for pPro and T7 expression systems Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.140 44000
-
 recombinant protein, gel filtration and SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.140 S-adenosyl-L-methionine + (S)-coclaurine Escherichia coli isoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.140 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.140 recombinant protein, gel filtration Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.140 culture supernatant
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.140 additional information
-
 analysis of inducible expression systems, production of recombinant CMT protein from PprpB promoter 2fold to 4fold higher than that from PT7 promoter, cell growth not reduced after expression form PprpB promoter even when highly induced, propionate-inducible system efficient for protein overproduction that result in growth inhibition, auto-induction experiments performed and protein production monitored Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.140 S-adenosyl-L-methionine + (S)-coclaurine isoquinoline alkaloid biosynthesis Escherichia coli S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (S)-coclaurine expression systems tested by analyzing recombinant CMT protein Escherichia coli S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.140 CMT
-
 Escherichia coli
2.1.1.140 coclaurine N-methyltransferase
-
 Escherichia coli